905429
TissueFab® bioink
(Gel)ma -UV/365 nm
Synonym(s):
Bioink, GelMA, Gelatin methacrylamide, Gelatin methacrylate, Gelatin methacryloyl
Sign Into View Organizational & Contract Pricing
All Photos(3)
About This Item
UNSPSC Code:
12352201
NACRES:
NA.23
Recommended Products
description
0.2 μm sterile filtered, suitable for 3D bioprinting applications
form
viscous liquid
impurities
≤5 CFU/g Bioburden (Fungal)
≤5 CFU/g Bioburden (Total Aerobic)
color
colorless to pale yellow
pH
6.5-7.5
application(s)
3D bioprinting
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
General description
3D bioprinting is the printing of biocompatible materials, cells, growth factors and the other supporting materials necessary to yield functional complex living tissues. 3D bioprinting has been used to generate several different types of tissue such as skin, bone, vascular grafts, and cartilage structures. Based upon the desired properties, different materials and formulations can be used to generate both hard and soft tissues. While several 3D printing methods exist, due to the sensitivity of the materials used, extrusion-based methods with bioinks are most commonly employed.
Application
Gelatin methacryloyl (GelMA) is a polymerizable hydrogel material derived from natural extracellular matrix (ECM) components. Due to its low cost, abundance, and retention of natural cell binding motifs, gelatin has become a highly sought material for tissue engineering applications. The addition of photocrosslinkable methacrylamide functional groups in GelMA allows the synthesis of biocompatible, biodegradable, and non-immunogenic hydrogels that are stable in biologically relevant conditions and promote cell adhesion, spreading, and proliferation. In addition to fast gelation, the methacrylamide functional group can also be used to control the hydrogel physical parameters such as pore size, degradation rate, and swell ratio. Temporal and spatial control of the crosslinking reaction can be obtained by adjusting the degree of functionalization and polymerization conditions, allowing for the fabrication of hydrogels with unique patterns, 3D structures, and morphologies. Gelatin methacrylate based bioinks have been used to bioprint osteogenic, chondrogenic, hepatic, adipogenic, vasculogenic, epithelial, endothelial, cardiac valve, skin, tumor and other tissues and constructs.
Packaging
Product contains 10ml of solution packaged in glass bottle.
Other Notes
Important tips for optimal bioprinting results
Procedure
1. Prepare bioink solution: Warm TissueFab® - GelMA-UV bioink in a water bath or incubator set to 37 °C for 30 minutes or until the bioink becomes fluid. Gently invert the bioink to make a homogeneous solution. DO NOT vortex or shake vigorously.
2. Prepare bioink-cell solution: Resuspend the cell pellet at the desired cell density with the bioink solution by gently pipetting up and down. Typical cell density for extrusion-based bioprinting is 1 to 5 x 106 cells/mL. Load the bioink-cell solution into the desired printer cartridge.
3. Bioprint: Cool the filled printing cartridge to 15–19 °C to induce gelation, using a temperature controlled printhead or place the cartridge in at 4 °C for 10–15 minutes. If print bed temperature control is available, set temperature to 15 °C. Follow the 3D printer manufacturer′s instructions. Load the print cartridge onto the 3D printer and print directly onto a Petri dish or into multi-well plates. Adjust the flow according to nozzle diameter, printing speed, printing pressure, and temperature.
4. Crosslink: Place the UV light source directly above the 3D-bioprinted structure and expose the structure to UV light (wavelength 365 nm). Use the appropriate distance settings and exposure times for your bioprinter.
5. Culture cells: Culture the bioprinted tissue with appropriate cell culture medium following standard tissue culture procedures.
- Optimize printing conditions (e.g., nozzle diameter, printing speed, printing pressure, temperature, cell density) for the features of your 3D printer and your application.
- Reduce bubble formation. Air bubbles in bioink may hamper bioprinting. Carefully handle the bioink when you mix and transfer it to avoid bubble formation. Do not vortex or shake vigorously.
- UV light Crosslinking. Position the light source directly above the printed structure. Lower intensity light sources will require shorter distances and longer exposure times to complete crosslinking. Recommended conditions: Place an 800 mW/cm2 light source 8 cm above the printed structure and expose for 30 s.
Procedure
1. Prepare bioink solution: Warm TissueFab® - GelMA-UV bioink in a water bath or incubator set to 37 °C for 30 minutes or until the bioink becomes fluid. Gently invert the bioink to make a homogeneous solution. DO NOT vortex or shake vigorously.
2. Prepare bioink-cell solution: Resuspend the cell pellet at the desired cell density with the bioink solution by gently pipetting up and down. Typical cell density for extrusion-based bioprinting is 1 to 5 x 106 cells/mL. Load the bioink-cell solution into the desired printer cartridge.
3. Bioprint: Cool the filled printing cartridge to 15–19 °C to induce gelation, using a temperature controlled printhead or place the cartridge in at 4 °C for 10–15 minutes. If print bed temperature control is available, set temperature to 15 °C. Follow the 3D printer manufacturer′s instructions. Load the print cartridge onto the 3D printer and print directly onto a Petri dish or into multi-well plates. Adjust the flow according to nozzle diameter, printing speed, printing pressure, and temperature.
4. Crosslink: Place the UV light source directly above the 3D-bioprinted structure and expose the structure to UV light (wavelength 365 nm). Use the appropriate distance settings and exposure times for your bioprinter.
5. Culture cells: Culture the bioprinted tissue with appropriate cell culture medium following standard tissue culture procedures.
Legal Information
TISSUEFAB is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Jun Yin et al.
ACS applied materials & interfaces, 10(8), 6849-6857 (2018-02-07)
Methacrylated gelatin (GelMA) has been widely used as a tissue-engineered scaffold material, but only low-concentration GelMA hydrogels were found to be promising cell-laden bioinks with excellent cell viability. In this work, we reported a strategy for precise deposition of 5%
Christine McBeth et al.
Biofabrication, 9(1), 015009-015009 (2017-01-11)
Due to its relatively low level of antigenicity and high durability, titanium has successfully been used as the major material for biological implants. However, because the typical interface between titanium and tissue precludes adequate transmission of load into the surrounding
Wanjun Liu et al.
Advanced healthcare materials, 6(12) (2017-05-04)
Bioprinting is an emerging technique for the fabrication of 3D cell-laden constructs. However, the progress for generating a 3D complex physiological microenvironment has been hampered by a lack of advanced cell-responsive bioinks that enable bioprinting with high structural fidelity, particularly
Methacrylated gelatin and mature adipocytes are promising components for adipose tissue engineering.
Birgit Huber et al.
Journal of biomaterials applications, 30(6), 699-710 (2015-05-29)
In vitro engineering of autologous fatty tissue constructs is still a major challenge for the treatment of congenital deformities, tumor resections or high-graded burns. In this study, we evaluated the suitability of photo-crosslinkable methacrylated gelatin (GM) and mature adipocytes as components
Y Shi et al.
Biomedical materials (Bristol, England), 13(3), 035008-035008 (2018-01-09)
Three-dimensional bioprinting is an emerging technology for fabricating living 3D constructs, and it has shown great promise in tissue engineering. Bioinks are scaffold materials mixed with cells used by 3D bioprinting to form a required cell-laden structure. In this paper
Articles
Bioinks enable 3D bioprinting of tissue constructs for drug screening and transplantation; select suitable bioinks for specific tissue engineering.
Bioinks enable 3D bioprinting of tissue constructs for drug screening and transplantation; select suitable bioinks for specific tissue engineering.
Bioinks enable 3D bioprinting of tissue constructs for drug screening and transplantation; select suitable bioinks for specific tissue engineering.
Bioinks enable 3D bioprinting of tissue constructs for drug screening and transplantation; select suitable bioinks for specific tissue engineering.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service