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803103

Sigma-Aldrich

25-Hydroxyvitamin D3-23,24,25,26,27-13C5 solution

100 μg/mL in ethanol, 99 atom % 13C, 95%

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About This Item

Empirical Formula (Hill Notation):
13C5C22H44O2
CAS Number:
19356-17-3
Molecular Weight:
405.60
UNSPSC Code:
12352205
PubChem Substance ID:
329768779
NACRES:
NA.12

isotopic purity

99 atom % 13C

Quality Level

200

Assay

95%

form

liquid

concentration

100 μg/mL in ethanol

technique(s)

mass spectrometry (MS): suitable

color

colorless

application(s)

detection

mass shift

M+5

storage temp.

−20°C

SMILES string

[13CH3][13C](O)([13CH3])[13CH2][13CH2]C[C@@H](C)C1CCC([C@]1(C)CCC/2)C2=C\C=C(C[C@@H](O)CC3)/C3=C

InChI

1S/C27H44O2/c1-19-10-13-23(28)18-22(19)12-11-21-9-7-17-27(5)24(14-15-25(21)27)20(2)8-6-16-26(3,4)29/h11-12,20,23-25,28-29H,1,6-10,13-18H2,2-5H3/b21-11+,22-12-/t20-,23+,24?,25?,27-/m1/s1/i3+1,4+1,6+1,16+1,26+1

InChI key

JWUBBDSIWDLEOM-UZYJFTCKSA-N

General description

25-Hydroxyvitamin D3 (calcifediol) is a prohormone produced via hydroxylation of vitamin D3 (cholecalciferol) in the liver. It is a precursor for the synthesis of calcitriol {1,25-dihydroxyvitamin D3 or [1,25(OH)2D3]}. It is used as a biomarker to determine the status of vitamin D in the body.
25-Hydroxyvitamin D3-23,24,25,26,27-13C5 is an isotope of vitamin D3 wherein C-23, C-24, C-25, C-26, C-27 carbons are replaced by 13C6 isotope.

Application

25-Hydroxyvitamin D3-23,24,25,26,27-13C5 can be used as a stable isotope internal standard for data interpretation in specific biological samples by mass spectrometry.

Packaging

1 mL in ampule
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

57.2 °F

Flash Point(C)

14 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Need of a dedicated isotopic internal standard for accurate 3-epi-25 (OH) D3 quantification by LC-MS/MS
Zakaria R, et al.
Clinical Chemistry and Laboratory Medicine, 57(7), e141-e144 (2019)
Quantification of circulating 25-hydroxyvitamin D by liquid chromatography-tandem mass spectrometry
Vogeser M
The Journal of Steroid Biochemistry and Molecular Biology, 121(3-5), 565-573 (2010)
25-Hydroxyvitamin D3, the prohormone of 1, 25-dihydroxyvitamin D3, inhibits the proliferation of primary prostatic epithelial cells
Barreto, AM et al.
Cancer Epidemiology, Biomarkers & Prevention, 9(3), 265-270 (2000)

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