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Key Documents

746622

Sigma-Aldrich

SAM Formation reagent for biotin terminated monolayer on gold surface

Synonym(s):

Biotin-SAM

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About This Item

UNSPSC Code:
12352103
NACRES:
NA.23

form

solid

Quality Level

100

storage temp.

2-8°C

Related Categories

Application

Because the Biotin-Avidin formation is known as a facile reaction, this product is widely used to immobilize a protein on a Surface Plasmon Resonance (SPR) or Quartz Crystal Microbalance (QCM) biosensor consisting of a Self-Assembled Monolayer (SAM).

Used for:
  • Simple preparation of Biotin-SAM on gold surface
  • Efficient Immobilization of Avidins
  • Suppress unspecific binding with unknown proteins

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCR1052
MKCQ1521
MKCQ1520
MKCN4730
MKCK3635

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Jennifer S Shumaker-Parry et al.
Analytical chemistry, 76(4), 918-929 (2004-02-14)
We present two strategies for microspotting 10 x 12 arrays of double-stranded DNAs (dsDNAs) onto a gold-coated glass slide for high-throughput studies of protein-DNA interactions by surface plasmon resonance (SPR) microscopy. Both methods use streptavidin (SA) as a linker layer
Yoshiko Ishizuka-Katsura et al.
Journal of bioscience and bioengineering, 105(5), 527-535 (2008-06-19)
We describe a technique to form a biotin-containing phospholipid vesicle layer on a self-assembled monolayer (SAM) deposited on a gold surface to immobilize biotinylated receptor proteins for a surface plasmon resonance (SPR) biosensor. The adsorption of vesicle of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)

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