201545

Fast Blue RR

• Synonym(s): 4-Benzoylamino-2,5-dimethoxyaniline, Azoic Diazo No. 24
• Empirical Formula (Hill Notation): C₁₅H₁₆N₂O₃
• CAS Number: 6268-05-9
• Molecular Weight: 272.30
• Colour Index Number: 37155
• EC Number: 228-441-6
• MDL number: MFCD00008369
• UNSPSC Code: 12171500
• PubChem Substance ID: 24852010
• NACRES: NA.47

Properties

• Assay: >96.5% (HPLC)
• Quality Level: 100
• form: solid
• mp: 165-168 °C (lit.)
• λmax: 323 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: COc1cc(NC(=O)c2ccccc2)c(OC)cc1N
• InChI: 1S/C15H16N2O3/c1-19-13-9-12(14(20-2)8-11(13)16)17-15(18)10-6-4-3-5-7-10/h3-9H,16H2,1-2H3,(H,17,18)
• InChI key: DDRCIGNRLHTTIW-UHFFFAOYSA-N

Description

Application

Fast Blue RR has been used to detect alkaline phosphatase activity.

Biochem/physiol Actions

Fast blue RR is mainly used in histological stainings. It binds to the reaction product of alkaline phosphatase and precipitates it as an insoluble compound. The sites of alkaline phosphatase activity appear black. Fast blue RR is also used for the detection of esterase activity. It couples with naphthol (released from naphthol acetate) and forms an insoluble azo-dye which appears red/blue.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315,H319,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable