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Key Documents

SAB4200785

Sigma-Aldrich

Anti-B2M antibody, Mouse monoclonal

clone 92A, purified from hybridoma cell culture

Synonym(s):

Anti-Beta-2-microglobulin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

92A, monoclonal

form

buffered aqueous solution

mol wt

~15 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

immunoblotting: 0.1-0.2 μg/mL using β2-Microglobulin from human urine.
immunoprecipitation (IP): 2.5-5 μg/test using lysate of human HeLa cell line.
indirect ELISA: 0.2-0.4 μg/mL using 1 μg/ml β2-Microglobulin from human urine for coating.

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... B2M(567)

General description

B2M (β2-Microglobulin) is the light chain of the major histocompatibility class (MHC) I molecule expressed on the cell surface of all nucleated cells, including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells and is absent in erythrocytes. B2M is filtered during glomerular filtrations and re-absorbed and catabolized by proximal renal tubule. While under normal conditions the B2M is undetectable in urine, its increased urinary excretion has been observed to be an early marker of tubular injury in a number of settings, including nephrotoxicant exposure, cardiac surgery and renal transplantation, preceding rises in serum creatinine by as many as 4-5 days. B2M may also serve as an early biomarker for AKI (Acute Kidney Injury). B2M plays a vital role in cell survival, proliferation and metastasis in various types of cancer. Its levels are also linked with multiple myeloma (MM) and non-Hodgkin′s lymphoma (NHL). NHL patients showing higher levels of B2M have a poor disease prognosis and a higher mortality risk. The protein has a predominantly β-pleated sheet structure that can form amyloid fibrils in some pathological conditions. In patient subjected to long-term hemodialysis, B2M is responsible for systemic amyloidosis. The levels of this protein are also altered in the cerebrospinal fluid of patients with neurological diseases, such as leptomeningeal metastasis, purulent meningitis, viral meningitis or encephalitis and neuroborreliosis. Over-expression of serum B2M is observed in hepatitis C virus (HCV) related chronic liver diseases and thus acts as a potential marker for the HCV disease progression towards cirrhosis and carcinoma.

Immunogen

Recombinant B2M protein.

Application

Immunoblotting: a working concentration of 0.1-0.2 μg/mL is recommended using β2-Microglobulin from human urine.

Immunoprecipitation: a working concentration of 2.5-5 μg/test is recommended using lysate of human HeLa cell line.

Indirect ELISA: a working concentration of 0.2-0.4 μg/mL is recommended using 1 μg/mL ß2-Microglobulin from human urine for coating.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.

Other Notes

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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