SAB3700036
Anti-Bovine IgG (H+L), F(ab′)2 fragment-Peroxidase antibody produced in rabbit
affinity isolated antibody, lyophilized powder
Synonym(s):
HRP
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
rabbit
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
lyophilized powder
species reactivity
bovine
technique(s)
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Related Categories
General description
IgG (immunoglobulin G) contributes to 10−20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50kDa and 25kDa respectively. Limited digestion using papain cleaves the antibody into three fragments, two of which are identical and contain the antigen-binding activity. They are known as fragment antigen binding (Fab) fragments. These fragments contain the light chains paired with the VH and CH1 domains of the heavy chains. The variable region of IgG antibody is specific to antigens and is highly conserved. IgG antibody is abundantly present in mother′s milk.
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Bovine IgG and Bovine Serum. No reaction was observed against Anti-Pepsin or Anti-Rabbit IgG F(c).
Immunogen
Bovine IgG whole molecule
Biochem/physiol Actions
IgG (immunoglobulin G) antibody provides protection against bacterial, fungal and viral infections. Maternal IgG is transferred to fetus through the placenta that is vital for the immune defense of the neonate against infections. IgG antibody has its function similar to IgM antibody in complement system activation. IgG is involved in hypersensitivity type II and type III. It helps in opsonization, complement fixation and cell-mediated cytotoxicity.
Physical properties
Antibody format: IgG F(ab′)2
Physical form
Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Reconstitution
Reconstitute with 500 μ;L deionized water (or equivalent).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Certificates of Analysis (COA)
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IgG subclasses and allotypes: from structure to effector functions
Vidarsson G, et al.
Frontiers in Immunology, 5(4), 520-520 (2014)
Natural autoantibodies
Antonio C, et al.
Current Opinion in Immunology, 7(6), 812-818 (1995)
Placental transfer of IgG subclasses in a Japanese population.
Hashira S, et al.
Pediatrics International, 42(4) (2000)
Gestur Vidarsson et al.
Frontiers in immunology, 5, 520-520 (2014-11-05)
Of the five immunoglobulin isotypes, immunoglobulin G (IgG) is most abundant in human serum. The four subclasses, IgG1, IgG2, IgG3, and IgG4, which are highly conserved, differ in their constant region, particularly in their hinges and upper CH2 domains. These
Specific IgG for cat allergens in patients with allergic conjunctivitis.
Miyama A, et al.
International Ophthalmology, 35(4), 575-586 (2015)
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