The control primers specific for the mouse GAPDH promoter are as follows:
FOR: 5'-CCTCTGCGCCCTTGAGCTAGGA-3’
REV: 5'-CACAAGAAGATGCGGCCGTCTC-3’
RIP
Imprint® RNA Immunoprecipitation Kit
High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA
Synonym(s):
Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation
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About This Item
NACRES:
NA.54
UNSPSC Code:
41116158
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Product Name
Imprint® RNA Immunoprecipitation Kit, High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA
Quality Level
Related Categories
1 of 4
This Item | CHP1 | 17-700 | 17-10521 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
Features and Benefits
- Xtra Protein A magnetic beads with higher binding capacity
- Step-by-step protocols for cell lysis and immunoprecipitation
- RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
- Negative control and antibody included
Application
Imprint® RNA Immunoprecipitation Kit has been used for:
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques[1]
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
General description
Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP.
Learn more about this product and view application data.
Learn more about this product and view application data.
RNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR.
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
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Hazard Classifications
Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
Storage Class
3 - Flammable liquids
wgk
WGK 3
flash_point_f
100.4 °F
flash_point_c
38 °C
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Instructions
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Please suggest the appropriate control primer set recommended for use with mouse tissue samples while using EZ Magna Chip A/G kit, 17-10086. It includes control antibodies (anti-RNA pol II) and a control primer set intended for the human GAPDH promoter.
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