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Key Documents

SW 1463

90112713, human colon, Epithelial

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About This Item

UNSPSC Code:
41106514

product name

SW 1463, 90112713

biological source

human colon

growth mode

Adherent

karyotype

2n = 46, hypertriploid

morphology

Epithelial

products

Carcinoembryonic antigen (CEA)

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian rectum adenocarcinoma

Cell Line Description

Established from a grade II mucous producing and grade III solid rectal adenocarcinoma from a 66 year old Caucasian female. The cells form brush borders and produce CEA.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12,13
D16S539: 11
D5S818: 13,14
D7S820: 9
THO1: 6,7
TPOX: 8,11
vWA: 16

Culture Medium

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split subconfluent cultures 1:2 using 0.25% trypsin or trypsin/EDTA; Medium change after 4-5 days. 37°C. No CO2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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