C3921
Creatinase from Pseudomonas sp.
recombinant, expressed in E. coli, lyophilized powder, 10-15 units/mg protein
Synonym(s):
Creatine Amidinohydrolase
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
lyophilized powder
specific activity
10-15 units/mg protein
mol wt
45 kDa
composition
Protein, ~70% biuret
storage temp.
2-8°C
Application
Creatine amidinohydrolase is a clinically important enzyme used in the determination of creatinine in blood and urine.
Biochem/physiol Actions
Creatinase is a homodimer with subunit molecular weights of approximately 45 kDa. It catalyzes the hydrolysis of creatine, in the presence of H2O, to form urea and sarcosine.
Unit Definition
One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 °C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Selective embolization in the management of arteriovenous fistula after renal allograft biopsy preserves renal allograft function.
Galip Guz et al.
International urology and nephrology, 37(1), 207-208 (2005-09-01)
M-L Lee
Pediatric cardiology, 26(6), 792-796 (2005-08-06)
I report on a 3-month-old infant with pulmonary atresia-intact ventricular septum and ventriculocoronary communication (VCC) who underwent percutaneous radiofrequency valvulotomy and valvuloplasty (RFVV). The patient's cardiac troponin-I, creatine kinase (CK), and myocardial fraction of (CK-MB) were elevated before RFVV and
Ashutosh Tiwari et al.
International journal of biological macromolecules, 44(5), 408-412 (2009-05-12)
A matrix made up of chitosan-SiO(2)-multiwall carbon nanotubes (CHIT-SiO(2)-MWCNTs) nanocomposite was fabricated to investigate the immobilization of creatine amidinohydrolase (CAH). CAH enzyme was covalently immobilized with the CHIT-SiO(2)-MWCNTs matrix using glutaraldehyde as a linker. The resulting CAH/CHIT-SiO(2)-MWCNTs biomatrix was characterized
Jill A O'Loughlin et al.
ASAIO journal (American Society for Artificial Internal Organs : 1992), 50(3), 253-260 (2004-06-03)
An alginate microcapsule was developed that contains three enzymes (urease, uricase, and creatininase) capable of effectively degrading urea, uric acid, and creatinine, which are elevated to pathologic levels in patients with kidney failure. The capsules were evaluated in vitro and
Catherine Trumel et al.
Veterinary clinical pathology, 33(3), 128-132 (2004-08-31)
Urine creatinine concentration often is used in ratios such as urine protein:creatinine to compensate for dilution or concentration of spot urine samples. The purpose of this study was to compare the accuracy of different techniques of urine creatinine measurement currently
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