QIA92

Caspase Detection Kit (Red-VAD-FMK)

• UNSPSC Code: 41116133

Properties

• usage: sufficient for 100 tests
• Quality Level: 100
• species reactivity (predicted by homology): mammals
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• technique(s): flow cytometry: suitable
• input: sample type intact cells
• fluorescence: λ_ex ~540 nm; λ_em ~570 nm
• detection method: fluorometric
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A convenient and sensitive method for the detection of activated caspases in living cells. The fluorescent marker is VAD-FMK conjugated to sulforhodamine. This cell-permeable, non-toxic inhibitor binds irreversibly to activated caspases in apoptotic cells. The fluorescence intensity can be measured by fluorescence microscopy, fluorescence microplate reader or flow cytometry.

The activation of caspases plays a central role in apoptosis. The Caspase Detection Kit provides a convenient and sensitive means for detecting activated caspases in situ in living cells. The assay utilizes a caspase inhibitor (VAD-FMK) conjugated to sulfo-rhodamine as the fluorescent in situ marker. Red-VAD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspases in apoptotic cells. The red fluorescence label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.

Specificity

A broad range of species

Components

Labeled Caspase Inhibitor (Red-VAD-FMK), Wash Buffer, Caspase Inhibitor (Z-VAD-FMK), and a user protocol

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Specifications

Assay Time: 1.5 h

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids