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MAB5268

Sigma-Aldrich

Anti-Chromogranin A Antibody, clone LK2H10

clone LK2H10, Chemicon®, from mouse

Synonym(s):

CgA, Pituitary Secretory Protein I

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

clone

LK2H10, monoclonal

species reactivity

porcine, pig, monkey, rat, human, mouse

should not react with

guinea pig, rabbit, sheep

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CHGA(1113)

General description

Chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Specificity

Chromogranin, an acidic protein having a molecular weight of about 68 kDa abides in the polypeptide-hormone-containing secretory granules of human endocrine tissues. Anti-chromogranin A can be used to stain most normal and neoplastic endocrine tissues containing secretory granules (exceptions include cells from the hypothalamus and posterior pituitary, which are known to contain secretory granules). The amount of staining is usually proportional to the number of secretory granules present (1).

Application

Detect Chromogranin A using this Anti-Chromogranin A Antibody, clone LK2H10 validated for use in IH, IH(P) & WB.
Immunohistochemistry:
A 1-10 μg/mL concentration of a previous lot was used in IH.

Optimal working dilutions must be determined by end user.

Packaging

500 μL

Quality

Routinely evaluated by Western Blot on Mouse Pancreas lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected Chromogranin A on 10 μg of Mouse Pancreas lysate.

Target description

50 kDa

Linkage

Replaces: CBL219

Physical form

Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.05M Borate buffered saline, 0.15M NaCl, pH 8.2 with 0.1% sodium azide.

Analysis Note

Control
Pancreas, adrenal gland, bowel and thyroid tissues Immunoblot: PC12 cell lysate, human pancreatic tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tieying Hou et al.
Cancer cytopathology, 128(10), 725-732 (2020-06-24)
Insulinoma-associated protein 1 (INSM1) has recently emerged as a reliable nuclear immunostaining marker for detecting neuroendocrine tumors (NETs) in paraffin-embedded surgical samples and cytologic cell blocks, but the reliability of INSM1 staining on cytologic smears is understudied. This study investigated
A transplantable human medullary thyroid carcinoma as a model for RET tyrosine kinase-driven tumorigenesis.
Johanson, V; Ahlman, H; Bernhardt, P; Jansson, S; Kolby, L; Persson, F; Stenman, G; Sward et al.
Endocrine-Related Cancer null
Malin Grönberg et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 56(9), 793-801 (2008-05-14)
Obestatin and ghrelin are two peptides derived from the same prohormone. It is well established that ghrelin is produced by endocrine cells in the gastric mucosa. However, the distribution of human obestatin immunoreactive cells is not thoroughly characterized. A polyclonal
Mark C Markowski et al.
The Prostate, 78(13), 992-1000 (2018-06-01)
Loss or mutation of PTEN alleles at 10q23 in combination with 8q24 amplification (encompassing MYC) are common findings in aggressive, human prostate cancer. Our group recently developed a transgenic murine model of prostate cancer involving prostate-specific Pten deletion and forced
Specific endocrine tissue marker defined by a monoclonal antibody.
Lloyd, R V and Wilson, B S
Science (New York, N.Y.), 222, 628-630 (1983)

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