MAB5254
Anti-Neurofilament 160 kDa Antibody, clone NN18
clone NN18, Chemicon®, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
NN18, monoclonal
species reactivity
guinea pig, chicken, rat, mouse, pig
species reactivity (predicted by homology)
human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
chicken ... Nefm(396206)
human ... NEFM(4741)
mouse ... Nefm(18040)
rat ... Nefm(24588)
General description
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.
Specificity
The antibody reacts with neurofilament 160 kD (Debus, E., 1982; Shaw, G., 1984).
Immunogen
Purified neurofilament polypeptides.
Application
Anti-Neurofilament 160 kDa Antibody, clone NN18 is an antibody against Neurofilament 160 kDa for use in IH, IH(P) & WB.
Immunohistochemistry:
5-10 µg/mL on frozen or paraffin embedded tissue. See protocols for details.
Western Blot:
1:1000. 7.5%-10% SDS-PAGE
Optimal working dilutions and protocols must be determined by end user.
5-10 µg/mL on frozen or paraffin embedded tissue. See protocols for details.
Western Blot:
1:1000. 7.5%-10% SDS-PAGE
Optimal working dilutions and protocols must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Quality
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Neurofilament on 10 μg of PC12 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Neurofilament on 10 μg of PC12 lysates.
Target description
160 kDa
Linkage
Replaces: CBL214
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Storage and Stability
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Do not freeze.
Do not freeze.
Analysis Note
Control
Brain
Cultured neurons
Brain
Cultured neurons
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lori Blanchfield et al.
Journal of immunology (Baltimore, Md. : 1950), 199(8), 2680-2691 (2017-09-10)
Of interest to the etiology of demyelinating autoimmune disease is the potential to aberrantly activate CD4+ T cells due to cross-recognition of multiple self-epitopes such as has been suggested for myelin oligodendrocyte glycoprotein epitope 35-55 (MOG35-55) and neurofilament medium protein
Sensory-motor deficits and neurofilament disorganization in gigaxonin-null mice.
Ganay, T; Boizot, A; Burrer, R; Chauvin, JP; Bomont, P
Mol. Neurodegener. null
Adrianne G Huxtable et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 30(11), 3947-3958 (2010-03-20)
Glia modulate neuronal activity by releasing transmitters in a process called gliotransmission. The role of this process in controlling the activity of neuronal networks underlying motor behavior is unknown. ATP features prominently in gliotransmission; it also contributes to the homeostatic
Chuang-Yu Lin et al.
JCI insight, 4(18) (2019-09-20)
The control of voluntary skeletal muscle contraction relies on action potentials, which send signals from the motor neuron through the neuromuscular junction (NMJ). Although dysfunction of the NMJ causes various neuromuscular diseases, a reliable in vitro system for disease modeling
Keith A Wharton et al.
PloS one, 11(5), e0155897-e0155897 (2016-05-19)
Over half of adults are seropositive for JC polyomavirus (JCV), but rare individuals develop progressive multifocal leukoencephalopathy (PML), a demyelinating JCV infection of the central nervous system. Previously, PML was primarily seen in immunosuppressed patients with AIDS or certain cancers
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