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Key Documents

MAB2259

Sigma-Aldrich

Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7

clone 1A7, from mouse

Synonym(s):

Cytochrome P450 46A1, cholesterol 24-hydroxylase, cytochrome P450, family 46, cytochrome P450, family 46, subfamily A, polypeptide 1, cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1A7, monoclonal

species reactivity

mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology), human (based on 100% sequence homology), rabbit (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_006659

UniProt accession no.

Q9Y6A2

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CYP46A1(10858)

General description

In enzymology, a cholesterol 24-hydroxylase (EC 1.14.13.98) is an enzyme that catalyzes the chemical reaction:
cholesterol + NADPH + H+ + O2 --> (24S)-24-hydroxycholesterol + NADP+ + H2O. Cholesterol 24-hydroxylase, a cytochrome P450 (CYP46A1), is expressed at 100-fold higher levels in the brain than in the liver. This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. Studies indicate that cholesterol 24-hydroxylase constitutes a major tissue-specific pathway for cholesterol turnover in the brain (Lund, 2003).

Specificity

This antibody recognizes cholesterol-24 hydroxylase.

Immunogen

Epitope: unknown
His-tagged recombinant protein corresponding to human cholesterol-24 hydroxylase.

Application

Detect Cholesterol-24 Hydroxylase using this Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7 validated for use in WB, IP, IC, IH.
Research Category
Neuroscience
Research Sub Category
Signaling Neuroscience

Quality

Evaluated by Western Blot in Cholesterol-24 hydroxylase mouse brain lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected Cholesterol-24 hydroxylase on 10 µg of Cholesterol-24 hydroxylase mouse brain lysate.

Target description

~ 54 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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H Benson Peng et al.
Biopharmaceutics & drug disposition, 42(8), 372-388 (2021-07-06)
Age, hypercholesterolemia, and vitamin D deficiency are risk factors that increase the brain accumulation of pathogenic β-amyloid peptides (40 and 42), precursors leading to Alzheimer's disease (AD) in humans. The relative changes accompanying aging, high cholesterol, and/or treatment of calcitriol
Fuxin Lu et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 41(2), 312-323 (2020-03-15)
The major pathway of brain cholesterol turnover relies on its hydroxylation into 24S-hydroxycholesterol (24S-HC) using brain-specific cytochrome P450 46A1 (CYP46A1). 24S-HC produced exclusively in the brain normally traverses the blood-brain barrier to enter the circulation to the liver for excretion;
Ahmed Haider et al.
Science translational medicine, 14(665), eadc9967-eadc9967 (2022-10-06)
Alterations in brain cholesterol homeostasis have been broadly implicated in neurological disorders. Notwithstanding the complexity by which cholesterol biology is governed in the mammalian brain, excess neuronal cholesterol is primarily eliminated by metabolic clearance via cytochrome P450 46A1 (CYP46A1). No

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