Skip to Content
Merck
All Photos(1)

Key Documents

AB2994

Sigma-Aldrich

Anti-Phospho-Bid (Ser65) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

BH3-interacting domain death agonist, p22 BID, BID

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pSer65)

Gene Information

human ... BID(637)

Related Categories

General description

Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.

Specificity

Other species have not been tested.
The antibody reacts with the 22 kDa phospho-Bid-S65 protein.

Immunogen

Epitope: Ser65

Application

Anti-Phospho-Bid (Ser65) Antibody detects level of Phospho-Bid (Ser65) & has been published & validated for use in WB.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blot on Jurkat cell lysate treated with (+/-) insulin.

Western Blot Analysis:
A 1:100~500 dilution of this antibody detected phospho-Bid-S65 on jurkat cell lysate treated with (+/-) insulin.

Target description

22 kDa

Physical form

Antigen Affinity Purified
Purified in PBS with 0.09% NaN3.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Western Blot:: Jurkat cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service