U7752

Urease from Canavalia ensiformis (Jack bean)

• CAS Number: 9002-13-5
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• EC Number: 232-656-0
• NACRES: NA.54
• MDL number: MFCD00070858
• EC Number: 3.5.1.5 (BRENDA, IUBMB)
• Biological source: Canavalia ensiformis

Properties

• biological source: Canavalia ensiformis
• Quality Segment: 200
• form: solid
• mol wt: hexamer 545 kDa, trimer ~272 kDa
• contains: ~20% dithiothreitol
• suitability: suitable for marker for electrophoresis (non-denaturing PAGE)
• storage temp.: −20°C

Description

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Urease is a multi-subunit nickel-dependent metalloenzyme. It is found abundantly in fungi, yeast, plants, and bacteria.[1] It belongs to the superfamily of amidohydrolases and phosphotriesterases.[2] Urease is also found in leguminous seeds.[3]

Application

Urease from Canavalia ensiformis (Jack bean) has been used:

• as a component of the molecular mass standard in electrophoresis[4]
• as a component of the standard in size-exclusion chromatography[5]
• as a reference in native-polyacrylamide gel electrophoresis (PAGE)

Biochem/physiol Actions

Urease is an essential enzyme of the nitrogen cycle.[1] It plays a role in degrading urea into ammonia and carbamate.[1][2] Urease provides a source of nitrogen for growth to various organisms. It also plays a major role in seed germination and seed chemical defense.[3]

Other Notes

Contains approx. 1 mg protein

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1