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About This Item
NACRES:
NA.32
UNSPSC Code:
12352200
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storage temp.
2-8°C
Quality Level
1 of 4
This Item | FLAGIPT1 | F2426 | A2220 |
|---|---|---|---|
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
Application
The FLAG® HA Tandem Affinity Purification Kit is designed for the isolation of high purity FLAG-HA dual-tagged fusion proteins from complex matrix, such as cell lysates and tissue homogenates. The kit is particularly suitable for isolation of protein complexes using TAP (Tandem Affinity Purification) technology.
Sutitable for mass spectrometry analysis and western blotting.
Learn more product details in our FLAG® application portal.
Sutitable for mass spectrometry analysis and western blotting.
Learn more product details in our FLAG® application portal.
General description
TAP technology is a recent development in protein purification. This technology incorporates tandem-linked affinity tags into genes of interest so that high purity fusion proteins can be isolated through two consecutive affinity purification steps. The technology is particularly useful for the isolation and identification of protein complexes by adding a tandem affinity tag to a known "bait" protein to pull down endogenous proteins that interact with the targeted protein of interest.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2
Storage Class
10 - Combustible liquids
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Junsong Zhang et al.
Journal of virology, 90(8), 3966-3980 (2016-02-05)
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Wei Li et al.
Cell, 140(4), 477-490 (2010-02-25)
Current models imply that the FERM domain protein Merlin, encoded by the tumor suppressor NF2, inhibits mitogenic signaling at or near the plasma membrane. Here, we show that the closed, growth-inhibitory form of Merlin accumulates in the nucleus, binds to
Karthik Ramachandran et al.
iScience, 25(1), 103722-103722 (2022-01-11)
SARS-CoV-2 is a newly identified coronavirus that causes the respiratory disease called coronavirus disease 2019 (COVID-19). With an urgent need for therapeutics, we lack a full understanding of the molecular basis of SARS-CoV-2-induced cellular damage and disease progression. Here, we
Kosei Sato et al.
Nature communications, 10(1), 166-166 (2019-01-13)
In Drosophila, some neurons develop sex-specific neurites that contribute to dimorphic circuits for sex-specific behavior. As opposed to the idea that the sexual dichotomy in transcriptional profiles produced by a sex-specific factor underlies such sex differences, we discovered that the
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