T9950

Tau-352 human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE), lyophilized powder

• Synonym(s): Human Tau Protein
• MDL number: MFCD07785730
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥90% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 36.8 kDa
• UniProt accession no.: P10636
• application(s): cell analysis
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... MAPT(4137)

Description

Application

Tau-352 human is a Tau isoform, variant 0N3R which means it consists of 3 microtubule binding repeats (R) and no amino terminal inserts (N). The product can be utilized for systemic identification of Phosphorylation sites in microtubule-associated protein tau by using checkpoint kinases Chk1 and Chk2 in vitro.

Biochem/physiol Actions

Tau-383 human belongs to neuronal microtubule-associated protein family that stimulates the microtubule assembly and stability as well as facilitates hyperphosphorylation-induced self-assembly into filaments. Tau protein along with src can upregulate tyrosine kinase activity. Inhibition of Src kinase influences the action of tau on actin rearrangements which in turn impacts the growth-factor-induced actin remodeling.

Isoform of Tau, variant 0N3R, having 3 microtubule binding repeats (R) and no amino terminal inserts (N).

Reconstitution

Lyophilized from MES, pH 6.8, containing NaCl and EGTA. When reconstituted in water to a protein concentration of 1 mg/mL, the resulting buffer will have ~50 mM MES, pH 6.8, 100 mM NaCl, and 0.5 mM EGTA.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves