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Key Documents

SRP0416

Sigma-Aldrich

T4 Beta-glucosyltransferase

recombinant, expressed in E. coli, ≥83% (SDS-PAGE)

Synonym(s):

T4-phage beta-glucosyltransferase, UDP glucose-DNA ?-glucosyltransferase, hydroxymethyl cytosine

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.32

biological source

Escherichia coli

recombinant

expressed in E. coli

Assay

≥83% (SDS-PAGE)

form

aqueous solution

mol wt

41.6 kDa

packaging

pkg of 100 μg

NCBI accession no.

NP_049658

UniProt accession no.

P04547

shipped in

dry ice

storage temp.

−70°C

Gene Information

bacteriophage T4 ... B-GT(1258765)

General description

T4-phage β−glucosyltransferase, also known as UDP glucose-DNA β-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351(end) with C-terminal His-tag, MW= 41.6 kDa, expressed in Escherichia coli.

Application

Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.

Biochem/physiol Actions

T4-phage β-glucosyltransferase is involved in the transfer of glucose from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-HMC) in double-stranded DNA of the T4-phage. Ions such as Mg2+, Mn2+ and Ca2+ activate this enzyme.

Physical form

Formulated in 200 mM imidazole and 20% glycerol.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H360D

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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Glucosylation of deoxyribonucleic acid by enzymes from bacteriophage-infected Escherichia coli.
S R KORNBERG et al.
The Journal of biological chemistry, 236, 1487-1493 (1961-05-01)
Chun-Xiao Song et al.
Nature biotechnology, 29(1), 68-72 (2010-12-15)
In contrast to 5-methylcytosine (5-mC), which has been studied extensively, little is known about 5-hydroxymethylcytosine (5-hmC), a recently identified epigenetic modification present in substantial amounts in certain mammalian cell types. Here we present a method for determining the genome-wide distribution
High resolution crystal structures of T4 phage beta-glucosyltransferase: induced fit and effect of substrate and metal binding.
Morera S
Journal of Molecular Biology, 311(3), 569-577 (2001)
Luis A Ortega-Ramirez et al.
Antibiotics (Basel, Switzerland), 9(3) (2020-03-04)
The resistance of Escherichia coli O157:H7 to disinfection is associated with its ability to form biofilms, mainly constituted by glucans produced by glucosyltransferases. Citral and geraniol, terpenes found in the essential oil of Cymbopogon citratus (EO), have proven antibacterial activity
T4 phage beta-glucosyltransferase: substrate binding and proposed catalytic mechanism.
Morera S
Journal of Molecular Biology, 292(3), 717-730 (1999)
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