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SAB4501763

Sigma-Aldrich

Anti-HSL antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Hormone sensitive lipase, LIPE, LIPS

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 116 kDa

species reactivity

mouse, rat, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:5000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... LIPE(3991)

Related Categories

General description

Anti-HSL Antibody detects endogenous levels of total HSL protein.
Hormone sensitive lipase (HSL) is coded by LIPE (lipase E) gene. It is an intracellular lipase which acts on triglycerides, diglycerides, monoglycerides, cholesterol esters and retinyl esters. It is mapped to human chromosome 19q13.

Immunogen

The antiserum was produced against synthesized peptide derived from human HSL.

Immunogen Range: 518-567

Application

Anti-HSL antibody has been used in SDS-PAGE and western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Hormone sensitive lipase (HSL) plays an important role in lipolysis. Lack of HSL results in histologic abnormalities in white adipose tissue and an increase in macrophage infiltration.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Upregulation of Pnpla2 and Abhd5 and downregulation of G0s2 gene expression in mesenteric white adipose tissue as a potential reason for elevated concentration of circulating NEFA after removal of retroperitoneal, epididymal, and inguinal adipose tissue.
Dettlaff-Pokora A, et al.
Molecular and Cellular Biochemistry, 422(1-2), 21-29 (2016)
Siwen Yang et al.
Molecules (Basel, Switzerland), 24(1) (2018-12-26)
Despite the edible fungus Amillariella mellea possessing a variety of biological activities, its effects on diabetes are still unclear. Polysaccharides are the main bioactive ingredients. In order to destroy the cell wall to obtain more polysaccharides, we used NaOH solution
Progesterone-induced down-regulation of hormone sensitive lipase (Lipe) and up-regulation of G0/G1 switch 2 (G0s2) genes expression in inguinal adipose tissue of female rats is reflected by diminished rate of lipolysis
Stelmanska E, et al.
The Journal of Steroid Biochemistry and Molecular Biology, 147, 31-39 (2015)
Agnieszka Dettlaff-Pokora et al.
Molecular and cellular biochemistry, 422(1-2), 21-29 (2016-09-04)
Elevated concentrations of circulating non-esterified fatty acids (NEFA) were reported in (a) humans with lipodystrophy, (b) humans following bariatric surgery, and (c) transgenic mice with reduced amounts of adipose tissue. Paradoxically, these findings suggest that the reduction of adipose tissue
Null mutation in hormone-sensitive lipase gene and risk of type 2 diabetes
Albert JS, et al.
The New England Journal of Medicine, 370(24), 2307-2315 (2014)

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