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SAB4500620

Sigma-Aldrich

Anti-DAPK1 antibody produced in rabbit

affinity isolated antibody

Synonym(s):

DAP kinase 1, DAPK, Death associated protein kinase, kinase DAPK1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 160 kDa

species reactivity

mouse, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:1000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DAPK1(1612)

Related Categories

General description

Anti-DAPK1 Antibody detects endogenous levels of total DAPK1 protein.
Death associated protein kinase 1 (DAPK1) is a calcium/calmodulin–regulated (CaM-regulated) protein kinase that belongs to the kinases family. The protein structure contains a kinase domain, a CaM binding domain, eight ankyrin repeats, and a death domain. DAPK1 protein is encoded by the gene mapped to human chromosome 9q21.33. Anti-DAPK1 antibody detects endogenous levels of total DAPK1 protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human DAPK1.

Immunogen Range: 274-323

Application

Anti-DAPK1 antibody produced in rabbit has been used in:
  • immunohistochemistry(1:400 )
  • coimmunoprecipitation
  • immunoblot

Biochem/physiol Actions

Death associated protein kinase 1 (DAPK1) acts as a death-inducing factor in response to interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and transforming growth factor (TGF)-β. It also induces autophagy, proliferation, and immune response to inflammatory signals. DAPK1 promotes the growth of p53-mutant cancers, including pancreatic and ovarian cancers. Therefore, downregulation of DAPK1 can be a potential therapeutic strategy for treating P53 mutant cancers. Overexpression of DAPK1 protein has been observed in Alzheimer′s disease (AD) patients.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jing Zhang et al.
Cellular & molecular immunology, 11(3), 245-252 (2014-02-18)
Interferon regulatory factor (IRF) 7 has been demonstrated to be a master regulator of virus-induced type I interferon production (IFN), and it plays a central role in the innate immune response against viruses. Here, we identified death-associated protein kinase 1
Asia Asiaf et al.
Asian Pacific journal of cancer prevention : APJCP, 20(3), 839-848 (2019-03-27)
Aims: Death-associated protein kinase-1 (DAPK1) is a pro-apoptotic Ser/Thr kinase that participates in cell apoptosis and tumor suppression. DAPK1 is frequently lost in many different tumor types including breast cancer. The aim of this study was to evaluate the promoter
Craig Stevens et al.
The Journal of biological chemistry, 284(1), 334-344 (2008-11-01)
Death-associated protein kinase (DAPK) is a multidomain enzyme that plays a central role in autophagic and apoptotic signaling, although the protein-protein interactions regulating DAPK functions are not well defined. Peptide aptamer libraries were used to identify the tumor suppressor protein
Dongmei Chen et al.
Journal of pineal research, 69(2), e12665-e12665 (2020-05-03)
Death-associated protein kinase 1 (DAPK1) is upregulated in the brains of human Alzheimer's disease (AD) patients compared with normal subjects, and aberrant DAPK1 regulation is implicated in the development of AD. However, little is known about whether and how DAPK1 function
Jing Zhao et al.
The Journal of clinical investigation, 125(7), 2707-2720 (2015-06-16)
Estrogen receptor-negative (ER-negative) breast cancers are extremely aggressive and associated with poor prognosis. In particular, effective treatment strategies are limited for patients diagnosed with triple receptor-negative breast cancer (TNBC), which also carries the worst prognosis of all forms of breast

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