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Merck

RAB0802

Mouse IgG1 ELISA Kit

Synonym(s):

IgG1, Immunoglobulin G1

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About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Species reactivity:
mouse
Storage temp.:
−20°C
Shipped in:
wet ice
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species reactivity

mouse

technique(s)

ELISA: suitable

assay range

inter-assay cv: <10%
intra-assay cv: <12%

shipped in

wet ice

storage temp.

−20°C

General description

Immunoglobulin G (IgG) is the most prevalent protein found in the human serum. About 10–20% of plasma protein accounts for IgG. IgG1 is the major Ig isotype in murine serum. It resembles human IgG4 functionally. Murine immunoglobulin G1 (IgG1) is capable of blocking the complex formation of IgG2a, IgG2b, and IgG3 with C1q. C1q-mediated complement activation by IgG2a can be blocked by murine IgG1. Because of their likely inhibitory influence on both complement and classical Fcγ receptor (FcγR) activation, murine IgG1 may have the greatest inhibitory capacity toward all other IgG types. The antibody pair provided in this kit recognizes mouse IgG1.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

pictograms

Corrosion

signalword

Warning

hcodes

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Related Content


Gina-Maria Lilienthal et al.
Frontiers in immunology, 9, 958-958 (2018-06-06)
IgG antibodies (Abs) mediate their effector functions through the interaction with Fcγ receptors (FcγRs) and the complement factors. The main IgG-mediated complement activation pathway is induced through the binding of complement C1q to IgG Abs. This interaction is dependent on
Roxanne Collin et al.
Journal of immunology (Baltimore, Md. : 1950), 193(7), 3503-3512 (2014-08-29)
Autoimmune diseases result from a break in immune tolerance. Various mechanisms of peripheral tolerance can protect against autoimmunity, including immunoregulatory CD4(-)CD8(-) double-negative (DN) T cells. Indeed, we have previously shown that diabetes-prone mouse strains exhibit a low proportion of DN
Richard T Strait et al.
Nature, 517(7535), 501-504 (2014-11-05)
Immunoglobulins protect against disease to a considerable extent by activating complement and stimulatory immunoglobulin crystallizable fragment receptors (Ig FcRs), and aggregating microbial pathogens. Yet IgG1, the predominant murine serum Ig isotype, cannot activate complement by the classical pathway, binds more



Global Trade Item Number

SKUGTIN
RAB0802-1KT04061835165797