P3296

Protein G Sepharose^™, Fast Flow

recombinant, expressed in E. coli, aqueous ethanol suspension

• Synonym(s): Protein G-Agarose, Fast Flow from Streptococcus sp.
• UNSPSC Code: 41106500
• NACRES: NA.56
• MDL number: MFCD00165580
• Form: aqueous ethanol suspension
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• Quality Segment: 200
• form: aqueous ethanol suspension
• analyte chemical class(es): proteins (Immunoglobulins of various mammalian species)
• extent of labeling: ~2 mg per mL
• technique(s): affinity chromatography: suitable
• matrix: Sepharose 4B Fast Flow
• matrix activation: cyanogen bromide
• matrix attachment: amino
• matrix spacer: 1 atom
• storage temp.: 2-8°C

Description

General description

Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148.[1] This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200.[2] The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10.[3] It acts as a powerful reagent for the detection of IgG.[1]

P3296-5Ml′s updated product number is GE17-0618-01

Application

Protein G-Sepharose^™ is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, protein A, G and L resins, protein interaction, and purification and detection. Protein G-Sepharose^™ has been used to develop a strategy to confirm the presence of anti-erythropoietin neutralizing antibodies in human serum as well as to compare methods for depletion of albumin and IgG from equine serum.

Physical form

Suspension in 20% ethanol

Preparation Note

Prepared with recombinant streptococcal Protein G from which the albumin-binding region has been genetically deleted

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class: 3 - Flammable liquids
• wgk: WGK 3
• flash_point_f: 115.0 °F - closed cup
• flash_point_c: 46.1 °C - closed cup