P0609

Pepsin−Agarose from porcine gastric mucosa

lyophilized powder, ≥30 units/mg dry solid

• Synonym(s): Pepsin A
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 3.4.23.1 (BRENDA, IUBMB)
• MDL number: MFCD00081840
• Specific activity: ≥30 units/mg dry solid
• Biological source: Porcine gastric mucosa

Properties

• biological source: Porcine gastric mucosa
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥30 units/mg dry solid
• mol wt: 35 kDa
• matrix: 4% cross-linked beaded agarose
• UniProt accession no.: P00791
• storage temp.: −20°C
• Gene Information: pig ... LOC396892(396892)

Description

General description

Pepsin is an enzyme found in gastric secretion.

Application

Pepsin immobilized onto agarose beads can be used to digest and then separate out the active peptides of the glycoprotein gliadin.

Pepsin-agarose has been used as an immobilization tool to investigate the refolding ability of the pepsin enzyme. It has also been used for the preparation of F(ab′)2 fragments from rabbit IgG.

Used to produce F(ab′)₂ fragments of antibodies.¹

Biochem/physiol Actions

Preferentially cleaves C-terminal to Phe, Leu and Glu. It does not cleave at Val, Ala or Gly. pH optimum 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH > 6.

Physical form

Lyophilized powder stabilized with lactose

Preparation Note

One gram solid swells to 5-10ml packed gel.

Other Notes

One unit will produce a ΔA₂₈₀ of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P304 + P340 - P342 + P311 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves