The solvent used to prepare this proprietary Phosphatase Inhibitor Cocktail is 100% DMSO. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/306/088/p0044dat-ms.pdf
P0044
Phosphatase Inhibitor Cocktail 3
DMSO solution, for the inhibition of protein phosphatase 2A, alkaline phosphatases, protein phosphatases 1 and 2A, DMSO solution
Synonym(s):
Phosphatase inhibitor
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.77
EC Number:
200-664-3
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
2-8°C
Pricing and availability is not currently available.
Product Name
Phosphatase Inhibitor Cocktail 3, DMSO solution
form
DMSO solution
Quality Level
solubility
water: soluble
shipped in
wet ice
storage temp.
2-8°C
Application
The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been tested on extract from animal tissues including human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells. It has been optimized and tested for L-isozymes of alkaline phosphatase as measured with pNPP and for serine-threonine protein phosphatases.
Biochem/physiol Actions
This mixture contains individual components with specific inhibitory properties. Cantharidin inhibits protein phosphatase 2A. (-)-p-Bromolevamisole oxalate inhibits L-isoforms of alkaline phosphatases. Calyculin A inhibits protein phosphatases 1 and 2A.
Preparation Note
This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.
Disclaimer
As supplied, this product is stable for four years at the recommended 2-8°C storage temperature.
1 of 1
This Item | |||
|---|---|---|---|
| form DMSO solution | form DMSO solution | form DMSO solution | form DMSO solution |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| shipped in wet ice | shipped in dry ice | shipped in wet ice | shipped in - |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility water: soluble |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| P0044-1ML | 04061838446459 |
| P0044-5ML | 04061838446466 |
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Wie ist die Ausgangskonzentration des DMSOs in der Originallösung?
1 answer-
Helpful?
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Does this reagent contain EDTA or EGTA or similar?
1 answer-
This product does not contain any chelating agents, including EDTA and EGTA.
Helpful?
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What's the concentration of this reagent?
1 answer-
The concentration of the individual components are proprietary. See below for the inhibitory properties of each:
• Cantharidin: inhibits protein phosphatase 2A (PP-2A)
• (–)-p-Bromolevamisole oxalate: inhibits L-isoforms of alkaline phosphatases
• Calyculin A: inhibits protein phosphatases 1 and 2A (PP-1 and PP-2A)Please see the link below to review the full product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/306/088/p0044dat-ms.pdfHelpful?
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Does this product contain any metal chelators?
1 answer-
This phosphatase inhibitor cocktail does not contain a chelator. A phosphatase inhibitor cocktail is not offered that includes EDTA at this time. Other protease inhibitor cocktails with chelators are offered.
Helpful?
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