N6530

N1 Medium Supplement (100×)

solution, sterile-filtered, suitable for cell culture

• Synonym(s): N1 Supplement
• NACRES: NA.77
• UNSPSC Code: 12352207

Properties

• sterility: sterile-filtered
• Quality Segment: 300
• form: solution
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• storage temp.: 2-8°C

Description

General description

N1 Medium Supplement (100×) can be used as a supplement to produce complete N1 neural cell growth media or closely related media.

Application

N1 Medium Supplement (100×) has been used as:

• a component of the proliferation medium for culturing oligodendrocyte-like cells and neuroblastoma cells
• a supplement in the Dulbecco′s modified Eagle medium (DMEM/F12) to culture human oligodendrocytes
• a component of the α-minimum essential medium (MEM) for culturing induced primary retinal pigment epithelium (ipRPE) monolayers

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Other Notes

Contains 0.5mg/ml recombinant human insulin, 0.5mg/ml human transferrin (partially iron-saturated), 0.5μg/ml sodium selenite, 1.6mg/ml putrescine, and 0.73μg/ml progesterone.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)