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N6530

Sigma-Aldrich

N1 Medium Supplement (100×)

solution, sterile-filtered, suitable for cell culture

Synonym(s):

N1 Supplement

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.77

sterility

sterile-filtered

form

solution

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

General description

N1 Medium Supplement (100×) can be used as a supplement to produce complete N1 neural cell growth media or closely related media.

Application

N1 Medium Supplement (100×) has been used as:
  • a component of the proliferation medium for culturing oligodendrocyte-like cells and neuroblastoma cells
  • a supplement in the Dulbecco′s modified Eagle medium (DMEM/F12) to culture human oligodendrocytes
  • a component of the α-minimum essential medium (MEM) for culturing induced primary retinal pigment epithelium (ipRPE) monolayers

Other Notes

Contains 0.5mg/ml recombinant human insulin, 0.5mg/ml human transferrin (partially iron-saturated), 0.5μg/ml sodium selenite, 1.6mg/ml putrescine, and 0.73μg/ml progesterone.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Miguel Flores-Bellver et al.
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Age-related macular degeneration (AMD) is a leading cause of blindness worldwide. Drusen are key contributors to the etiology of AMD and the ability to modulate drusen biogenesis could lead to therapeutic strategies to slow or halt AMD progression. The mechanisms
Ludmila A Voloboueva et al.
Investigative ophthalmology & visual science, 46(11), 4302-4310 (2005-10-27)
To determine whether (R)-alpha-lipoic acid (LA) protects cultured human fetal retinal pigment epithelial (hfRPE) cells against oxidative injury and identify the pathways that may mediate protection. Cultured hfRPE cells were pretreated with various concentrations of LA for 14 to 16
Lucia Lichvarova et al.
Scientific reports, 10(1), 12518-12518 (2020-07-23)
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Astrocytes are the most numerous glial cell type with important roles in maintaining homeostasis and responding to diseases in the brain. Astrocyte function is subject to modulation by microRNAs (miRs), which are short nucleotide strands that regulate protein expression in

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