M6159

α₂-Macroglobulin from human plasma

BioUltra, ≥98% (SDS-PAGE)

• Synonym(s): α₂-M
• CAS Number: 1631137-21-7
• UNSPSC Code: 12352202
• NACRES: NA.61
• MDL number: MFCD00130472

Properties

• biological source: human plasma
• Quality Segment: 200
• product line: BioUltra
• assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• mol wt: ~720 kDa (four glycoprotein subunits)
• composition: Protein, 15-30% biuret
• technique(s): inhibition assay: suitable
• solubility: water: soluble 10 mg protein/mL, clear, colorless
• UniProt accession no.: P01023
• storage temp.: −20°C
• Gene Information: human ... A2M(2)

Description

Application

α₂-Macroglobulin was used to study impaired lipoprotein receptor-mediated peripheral binding of plasma amyloid-β which is an early biomarker for mild cognitive impairment preceding Alzheimer′s disease.[1] It was also used in the control of the classical and the MBL pathway of complement activation.[2]

Inhibits all classes of endoproteases by forming a complex with the protease. When the protease cleaves the macroglobulin "bait" sequence, the macroglobulin rearranges and traps the protease.

Biochem/physiol Actions

α₂-Macroglobulin (α₂M) is a multifunctional protein that is a broad spectrum protease inhibitor. α₂M is a large homotetrameric glycoprotein (720 kDa) that is connected by disulfide-linked dimers which non-covalently interact to give the quaternary structure. α₂M is found in normal plasma at a concentration of 220-230 mg/dl accounting for 3-5% of the total plasma protein.[3]

α₂-Macroglobulin is found abundantly in plasma and interstitial fluids. The protease-α₂-M balance plays an important role in mediating inflammatory tissue destruction. Serum levels of α₂-M and protease-α₂-M complexes are increased in patients with sepsis, emphysema, periodontitis, rheumatoid arthritis, and other inflammatory diseases, and oxidant inactivation of α₂-M may contribute to tissue destruction during inflammation.

Serum levels of α₂-Macroglobulin (α₂-M) and protease-α₂-M complexes are increased in patients with sepsis, emphysema, periodontitis, rheumatoid arthritis and other inflammatory diseases. It is hypothesized that the oxidant inactivation of α₂-M contributes to tissue destruction in inflammation.

Packaging

Package size based on protein content

Physical form

Lyophilized from 0.02 M Tris, 0.13 M glycine, pH 8.0, and 0.08 M trehalose

Analysis Note

Plasma from each donor has been tested and found negative for antibody to HIV-1/HIV-2, antibody to HCV and HbSAg.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable