Yes, this product contains phenol red. Phenol red is included in most medium as a visual pH indicator. The medium should be changed when the color turns yellow (acidic) or purple (basic).
M0268
Minimum Essential Medium Eagle
With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
EMEM, MEM
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
Pricing and availability is not currently available.
form
powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.
Application
Minimum Essential Medium Eagle has been used:
- in the isolation and culture of primary hepatocytes
- to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
- to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells
Features and Benefits
- Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
- The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.
Preparation Note
Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.
Other Notes
Essentially the same as the original formula, except it is provided as a powder.
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This Item | |||
|---|---|---|---|
| form powder | form liquid | form liquid | form powder |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components HEPES: no | components phenol red: yes | components phenol red: yes | components NaHCO3: no |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
classic media, classical media, FBS | classic media, classical media, MEM, classic media, classical media, FBS | classic media, classical media, MEM, classic media, classical media, FBS | classic media, classical media, FBS, hybrid cells |
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Storage Class
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Does Product M0268, Minimum Essential Medium Eagle, contain phenol red?
1 answer-
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What is the formulation of Product M0268, Minimum Essential Medium Eagle?
1 answer-
The formulation for this product can be found on the product datasheet.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Does Product M0268, Minimum Essential Medium Eagle, have an expiration date?
1 answer-
Yes, this product has an expiration date of 36 months from the date of manufacture. The expiration date can be found on the Certificate of Analysis.
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Does Product M0268, Minimum Essential Medium Eagle, contain sodium bicarbonate?
1 answer-
No, this product does not contain sodium bicarbonate. It is recommended to add 2.2 g sodium bicarbonate per Liter of medium. Use either Product No. S5761 (powder) or S8761 (7.5% solution).
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