Key Documents

View All Documentation

5-(Iodoacetamido)fluorescein

Name: 5-(Iodoacetamido)fluorescein
Brand: SIGMA

≥90% purity (HPLC), powder

Synonym(s):

5-IAF

Select a Size

Change View

Pack Size	SKU	Availability	Price

About This Item

Empirical Formula (Hill Notation):

C₂₂H₁₄INO₆

CAS Number:

63368-54-7

Molecular Weight:

515.25

UNSPSC Code:

12171500

PubChem Substance ID:

329815467

NACRES:

NA.47

Beilstein/REAXYS Number:

6543244

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

5-(Iodoacetamido)fluorescein, ≥90% (HPLC)

Quality Segment

200

assay

≥90% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow to orange

solubility

DMSO: 5 mg/mL

fluorescence

λ_ex 492 nm; λ_em 515 nm (Mercaptoethanol adduct), λ_ex 493 nm; λ_em 522 nm (pH 9.2)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(NC(=O)CI)ccc45)c1

InChI

1S/C22H14INO6/c23-10-20(27)24-11-1-4-15-14(7-11)21(28)30-22(15)16-5-2-12(25)8-18(16)29-19-9-13(26)3-6-17(19)22/h1-9,25-26H,10H2,(H,24,27)

InChI key

UATCLPJEZJKNHE-UHFFFAOYSA-N

Description

General description

5-(Iodoacetamido)fluorescein is a thiol reactive fluorescein derivative.

Application

5-(Iodoacetamido)fluorescein is used for the synthesis of fluorescently labeled organelles (nuclei and nuclear matrices), proteins and peptides that include enzymes.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	D0531	F9551	F3651
Sigma-Aldrich I9271 5-(Iodoacetamido)fluorescein	Sigma-Aldrich D0531 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride	Sigma-Aldrich F9551 Fluorescein-5-EX N-hydroxysuccinimide ester	Sigma-Aldrich F3651 Fluorescein 5(6)-isothiocyanate
technique(s) titration: suitable	technique(s) -	technique(s) titration: suitable	technique(s) titration: suitable
color yellow to orange	color yellow to orange	color yellow to orange	color yellow to orange
fluorescence λ_ex 492 nm; λ_em 515 nm (Mercaptoethanol adduct), λ_ex 493 nm; λ_em 522 nm (pH 9.2)	fluorescence λ_ex 495 nm; λ_em 516 nm	fluorescence λ_ex 511-519 nm	fluorescence -
solubility DMSO: 5 mg/mL	solubility -	solubility DMSO or DMF: soluble	solubility acetone: 1 mg/mL, clear, light yellow to yellow
form powder	form powder	form powder	form powder
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Identification of the 5-iodoacetamidofluorescein reporter site on the Na,K-ATPase.

Tyson PA

The Journal of Biological Chemistry, 264, 726-726 (1989)

Multisite phosphorylation of the Saccharomyces cerevisiae filamentous growth regulator Tec1 is required for its recognition by the E3 ubiquitin ligase adaptor Cdc4 and its subsequent destruction in vivo.

Bao MZ

Eukaryotic Cell, 9, 31-31 (2010)

Cysteine oxidation of tau and microtubule-associated protein-2 by peroxynitrite: modulation of microtubule assembly kinetics by the thioredoxin reductase system.

Lisa M Landino et al.

The Journal of biological chemistry, 279(33), 35101-35105 (2004-06-09)

Alterations in the redox status of proteins have been implicated in the pathology of several neurodegenerative conditions including Alzheimer and Parkinson diseases. We report that peroxynitrite- and hydrogen peroxide-induced disulfides in the neuron-specific microtubule-associated proteins tau and microtubule-associated protein-2 are

View All Related Papers

Global Trade Item Number

SKU	GTIN
I9271-100MG	04061832343327
I9271-25MG	04061832343334

Questions

Reviews

No rating value