I4777

Interferon-γ from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IFN-γ
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD00131391

Properties

• biological source: mouse
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• mol wt: protein 15.6 kDa
• packaging: pkg of 0.1 mg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• color: white
• solubility: water: soluble 1.000 mL, clear, colorless
• UniProt accession no.: P01580
• storage temp.: −20°C
• Gene Information: mouse ... Ifng(15978)

Description

Application

Interferon-γ from mouse has been used:

• for in vitro stimulation of the BMDC (bone marrow derived dendritic cell), BMM (bone marrow-derived macrophages) and CD11c⁺ (CD11 antigen-like family member C) lung macrophages[1]
• for stimulation of murine macrophages to induce the production of nitrite and nitrate[2]
• to check CCAAT/enhancer binding protein β (C/EBPβ)-mediated changes in the expression of prostaglandin synthesis enzymes in neuroinflammation[3]

Biochem/physiol Actions

Ifng (interferon-γ) plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections.[4]

Physical form

Lyophilized from a 0.2 μm filtered buffered solution. Package size based on protein content.

Analysis Note

The biological activity of recombinant mouse IFN-γ is measured by its ability to inhibit the proliferation of murine WEHI-279 cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)