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IMDM

Name: IMDM
Brand: SIGMA

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217469

Pricing and availability is not currently available.

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Properties

Product Name

Iscove′s Modified Dulbecco′s Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma

Quality Segment

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
L-glutamine: no
glucose: yes

shipped in

ambient

storage temp.

2-8°C

Description

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Preparation Note

Supplement with 0.584 g/L L-glutamine

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1 of 1

This Item	51471C	I6529	I7633
Sigma-Aldrich I3390 Iscove′s Modified Dulbecco′s Medium	Sigma-Aldrich 51471C Iscove′s Modified Dulbecco′s Medium	SAFC I6529 Iscove′s Modified Dulbecco′s Medium	Sigma-Aldrich I7633 Iscove′s Modified Dulbecco′s Medium
form liquid	form liquid	form liquid	form powder
sterility sterile-filtered	sterility sterile	sterility sterile-filtered	sterility -
technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable, cell culture \| stem cell: suitable	technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable	technique(s) cell culture \| hybridoma: suitable, cell culture \| mammalian: suitable
components phenol red: yes L-glutamine: no glucose: yes	components phenol red: 15.93 mg/L NaHCO₃: 3024 mg/L L-glutamine: 582 mg/L HEPES: 5958 mg/L sodium pyruvate: 110 mg/L	components glucose: yes phenol red: yes NaHCO₃: yes L-glutamine: yes	components phenol red: yes glucose: yes L-glutamine: yes
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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