H3757

Hyaluronidase from bovine testes

Type VIII, lyophilized powder, 300-1,000 U/mg

• Synonym(s): Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• CAS Number: 37326-33-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 253-464-3
• MDL number: MFCD00131351
• EC Number: 3.2.1.35 (BRENDA, IUBMB)
• Specific activity: 300-1,000 U/mg
• Biological source: bovine testis

Properties

• biological source: bovine testis
• Quality Segment: 200
• conjugate: conjugate (Glucosaminoglycan)
• type: Type VIII
• form: lyophilized powder
• specific activity: 300-1,000 U/mg
• mol wt: ~55 kDa (four subunits of 14 kDa each)
• UniProt accession no.: Q5E985, Q8SQG8
• relevant disease(s): cancer
• storage temp.: −20°C
• Gene Information: cow ... HYAL1(515397), HYAL2(281838)

Description

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Preparation Note

Prepared from aseptic filled solution of Type I-S.

Other Notes

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)