H0262

HAT Media Supplement (50×) Hybri-Max^™

lyophilized powder, suitable for hybridoma, BioXtra

• Synonym(s): HAT Supplement, Hypoxanthine-Aminopterin-Thymidin (HAT) media supplement
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Product Name: HAT Media Supplement (50×) Hybri-Max^™, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma
• Quality Segment: 200
• sterility: γ-irradiated
• product line: BioXtra
• form: lyophilized powder
• technique(s): cell culture | hybridoma: suitable
• impurities: endotoxin, tested
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

HAT Media Supplement (50×) Hybri-Max^™ has been used:

• in the selection and validation of hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient clones
• in the generation of mAbs (monoclonal antibodies)
• in the luciferase-based bioassay (LUC) assay

Preparation Note

Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 0.4 μM aminopterin, 16 μM thymidine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H302,H360FD
• pcodes: P202 - P264 - P270 - P280 - P301 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Repr. 1B
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type P2 (EN 143) respirator cartridges