We were trying to use this as a protein standard and found it to be a heterogeneous mess. Not sure what all is in here, but its a lot more than betagalactosidase
G6008
β-Galactosidase from Escherichia coli
Grade VI, lyophilized powder, ≥250 units/mg protein
β-Galactosidase from Escherichia coli
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
β-D-Galactoside galactohydrolase, Lactase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
Specific activity:
≥250 units/mg protein
Biological source:
Escherichia coli
Pricing and availability is not currently available.
biological source
Escherichia coli
Quality Segment
type
Grade VI
form
lyophilized powder
specific activity
≥250 units/mg protein
mol wt
465 kDa
does not contain
BSA as extender
composition
Protein, ≥50% biuret
shipped in
wet ice
storage temp.
−20°C
General description
β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 Da each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.[1]
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Application
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop[4].
Physical form
Partially purified; contains Tris buffer salts, magnesium chloride, DL-dithiothreitol and 2-mercaptoethanol
Other Notes
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| biological source Escherichia coli | biological source - | biological source - | biological source - |
| specific activity ≥250 units/mg protein | specific activity ≥500 units/mg protein | specific activity ≥500 units/mg protein (biuret) | specific activity ≥2600 units/g |
| form lyophilized powder | form lyophilized powder | form aqueous glycerol suspension | form liquid |
| mol wt 465 kDa | mol wt 465 kDa | mol wt 465 kDa | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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