Skip to Content
Merck
All Photos(1)

Key Documents

E0521

Sigma-Aldrich

Anti-Estrogen Receptor (18-32) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 67 kDa

species reactivity

human

technique(s)

microarray: suitable
western blot: 1:3000 using MCF7 cultured cell line extract

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ESR1(2099)

General description

Estrogen is a sex hormone that is a key regulator of growth, differentiation and function of reproductive tissues, skeletal and cardiovascular systems. The effect of estrogen is mediated by two intracellular receptors, ERα and ERβ. Although the two receptors interact with similar ligands, have identical DNA-binding domains and have redundant roles in estrogen signaling, they show distinct expression pattern. On binding to the receptor, the estrogen-ER complex enters nucleus and regulates the transcription of specific genes. The biological effects of estrogen and ER is by upstream activation of the pathway by growth factors. Some pathways that activate the estrogen-ER pathway are those mediated by AP-1, IGF-1, EGF, MAPK and PI3K/Akt . Anti-Estrogen Receptor (18-32) antibody is specific for 18-32 amino acids of human estrogen receptor (67 kDa). The product is an IgG fraction prepared from pooled antisera.

Immunogen

synthetic peptide corresponding to amino acids 18-32 of the human estrogen receptor.

Application

Anti-Estrogen Receptor (18-32) antibody may be used for immunoblotting using extracts of MCF7 cells at a working dilution of 1:3000. It may also be used for dot blot.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

The multifaceted mechanisms of estradiol and estrogen receptor signaling.
J M Hall et al.
The Journal of biological chemistry, 276(40), 36869-36872 (2001-07-19)
Coregulator codes of transcriptional regulation by nuclear receptors.
M G Rosenfeld et al.
The Journal of biological chemistry, 276(40), 36865-36868 (2001-07-19)
J F Couse et al.
Endocrinology, 138(11), 4613-4621 (1997-11-05)
Until recently, only a single type of estrogen receptor (ER) was thought to exist and mediate the genomic effects of the hormone 17beta-estradiol in mammalian tissues. However, the cloning of a gene encoding a second type of ER, termed ERbeta
V Giguère et al.
Nature, 331(6151), 91-94 (1988-01-07)
The gonads and adrenal glands produce steroids classified into five major groups which include the oestrogens, progestins, androgens, glucocorticoids and mineralocorticoids. Gonadal steroids control the differentiation and growth of the reproductive system, induce and maintain sexual characteristics and modulate reproductive
Osman El-Maarri et al.
PloS one, 6(1), e16252-e16252 (2011-02-12)
Previously, we reported on inter-individual and gender specific variations of LINE-1 methylation in healthy individuals. In this study, we investigated whether this variability could be influenced by age or sex hormones in humans. To this end, we studied LINE-1 methylation

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service