DH82

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94062922, dog blood, Macrophage

• Synonym(s): DH82 Cell Line
• UNSPSC Code: 41106514

Properties

• biological source: dog blood
• packaging: tube of 5 μg 94062922-DNA-5UG; pkg of vial of cells 94062922-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Macrophage
• products: Not specified
• receptors: FC-gamma receptors
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Canine Monocyte-macrophage

Cell Line Description

Derived from a ten year old male golden retriever with malignant histiocytosis. The cells have a macrophage-like morphology and are able to phagocytose latex particles. They are positive for Fc-gamma receptors and are negative for Fc-mu and C3b receptors. DH82 cells do not produce IL-1.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculture Routine

Maintain cultures at 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells are prone to sticking and can be removed with 0.02% EDTA.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.