SW 48

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89012702, human colon, Epithelial

• UNSPSC Code: 41106514
• Biological source: human colon
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: 2n = 46
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Properties

• biological source: human colon
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 89012702-DNA-5UG, pkg of vial of cells 89012702-1VL
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Epithelial
• products: Carcinoembryonic antigen (CEA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Tumourigenicity studies

Biochem/physiol Actions

Human colon adenocarcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 11,12
D16S539: 11,13
D5S818: 10,14
D7S820: 9,10
THO1: 6,9.3
TPOX: 8
vWA: 18,20

The cells produce a low level of CEA: 0.6ng/1,000,000 cells in 10 days. Cells are tumorigenic in nude mice. Established from a grade IV ulcerating tumour encircling the bowel of an 82 year old Caucasian female. HLA = A32, A33, B27, B35

Preparation Note

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; No CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.