C9249
CryoSOfree™ DMSO-free Cryopreservation Medium
Synonym(s):
cell freezing medium
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
form
liquid
Quality Segment
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
cell culture | mammalian: suitable, cryopreservation: suitable
greener alternative category
, Aligned
shipped in
wet ice
storage temp.
2-8°C
General description
Cultured cells in a suspension can be cryopreserved with minimal damage from freezing and thawing steps. CryoSOfree™ DMSO-free cryopreservation medium preserves them with high viability and physiological activity. This medium is recommended for the preservation of stem cells, hepatocytes, tissue sample and a wide variety of cell types and established cell lines. CryoSOfree™ DMSO-free cryopreservation medium is a proprietary formulation, containing no DMSO nor animal proteins. It is ready-to-use and complete with no additives required.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of DMSO for cryopreservation of cell cultures. For more information see here.
Application
CryoSOfree is a chemically defined DMSO-free cryopreservation medium. It is animal component free and has been shown to preserve the potential of stem cell for differentiation.
Features and Benefits
- Preserves pluripotency of stem cells
- Low cytotoxicity
- Chemically defined, serum free, protein free
- Ready to use
The following cell lines have been recovered at ≥90% following cryopreservation with CryoSOfree:
L929, KB, MC3T3, B16F1, Colon26, CaCO2, HT1080, MG63
Preparation Note
1 ml of CyroSOfree is sufficient for 5x105- 5x106 cells.
Legal Information
CryoSOfree is a trademark of Sigma-Aldrich Co. LLC
signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
10 - Combustible liquids
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Related Content
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Cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), glycerol, ethylene glycol, and propylene glycol have been used for the cryopreservation of cells and tissues. DMSO is the most effective CPA but shows high cytotoxicity and can effect differentiation. ɛ-poly-L-lysine (PLL)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| C9249-100ML | 04061833530368 |