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C0549

Sigma-Aldrich

Chloroform:Isoamyl alcohol 24:1

suitable for nucleic acid purification

Synonym(s):

chloroform-isoamyl alcohol

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About This Item

UNSPSC Code:
12190000
NACRES:
NA.52

form

liquid

contains

0.6-1.0% ethanol as stabilizer

suitability

suitable for nucleic acid purification

application(s)

agriculture

General description

Chloroform:Isoamyl Alcohol 24:1 is an organic reagent used for DNA extraction and precipitation.

Application

Chloroform:Isoamyl alcohol 24:1 has been used:

  • to extract bacterial genomic DNA, DNA from Daphnia spp, and salmon sperms.
  • for a single DNA precipitation step for high-quality DNA extraction.
  • for protein extraction and RNAse treatment.
  • in phenolic oxidation to separate phenolics from the aqueous portion.
  • for RNA isolation from brown adipose tissue (BAT), inguinal white adipose tissue (iWAT), and epididymal adipose tissue (eWAT).
  • in modified cetyltrimethyl ammonium bromide (CTAB) method to extract total RNA from Impatiens balsamina.

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3

Target Organs

Central nervous system, Liver,Kidney

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

185.0 °F

Flash Point(C)

85 °C

Certificates of Analysis (COA)

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A modified SDS-based DNA extraction method from raw soybean
Xia YC, et al.
Bioscience Reports, 39(2), BSR20182271-BSR20182271 (2019)
A modified CTAB-based protocol for total RNA extraction from the medicinal plant Impatiens balsamina (Balsaminaceae) for next-generation sequencing studies.
FOONG LC, et al.
Journal of Separation Science, 46(2) (2017)
Defective insulin-stimulated GLUT4 translocation in brown adipocytes induces systemic glucose homeostasis dysregulation independent of thermogenesis in female mice
Picatoste B, et al.
Molecular Metabolism, 53 (2021)
Malte Thodberg et al.
Nucleic acids research, 47(4), 1671-1691 (2018-12-20)
Fission yeast, Schizosaccharomyces pombe, is an attractive model organism for transcriptional and chromatin biology research. Such research is contingent on accurate annotation of transcription start sites (TSSs). However, comprehensive genome-wide maps of TSSs and their usage across commonly applied laboratory
Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species
Healey A, et al.
Plant methods, 10(1), 1-8 (2014)
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