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C0412

Concanavalin A from Canavalia ensiformis (Jack bean)

Type IV-S, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

Synonym(s):

Con A

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About This Item

CAS Number:
11028-71-0
UNSPSC Code:
23151817
EC Number:
234-258-2
NACRES:
NA.32
MDL number:
MFCD00071069
Form:
lyophilized powder
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Quality Level

200

sterility

γ-irradiated

type

Type IV-S

product line

BioReagent

form

lyophilized powder

technique(s)

cell culture | mammalian: suitable

impurities

salt, essentially free

solubility

PBS: 5 mg/mL, slightly hazy, H2O: soluble, slightly hazy

storage temp.

−20°C

Application

Concanavalin A from Canavalia ensiformis (jack bean) has been used:
  • to induce spleen blastogenesis
  • to generate ConA blast targets from B6 and lpr spleen cells
  • to stimulate splenic lymphocytes from estrogen-and placebo-treated mice

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

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This Item
L7647C7642C2010
Concanavalin A from Canavalia ensiformis (Jack bean) Type IV-S, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

Sigma-Aldrich

C0412

Concanavalin A from Canavalia ensiformis (Jack bean)

Concanavalin A from Canavalia ensiformis (Jack bean) Type VI, lyophilized powder

Sigma-Aldrich

L7647

Concanavalin A from Canavalia ensiformis (Jack bean)

Concanavalin A from Canavalia ensiformis (Jack bean) FITC conjugate, Type IV, lyophilized powder

Sigma-Aldrich

C7642

Concanavalin A from Canavalia ensiformis (Jack bean)

Concanavalin A from Canavalia ensiformis (Jack bean) Type IV, lyophilized powder

Sigma-Aldrich

C2010

Concanavalin A from Canavalia ensiformis (Jack bean)

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

impurities

salt, essentially free

impurities

Carbohydrate, essentially free, salt, essentially free

impurities

Carbohydrate, essentially free

impurities

Carbohydrate, essentially free, salt, essentially free

solubility

PBS: 5 mg/mL, slightly hazy, H2O: soluble, slightly hazy

solubility

water: 1 % (w/v)

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

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pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000504211
0000504211
0000450266
0000450266
SLCP7735

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Global Trade Item Number

SKUGTIN
C0412-5MG04061833450598

Questions

  1. ConA 제품 (C0412)에 관해 질문 드립니다. ConA가 Ca2+, Mn2+ 이온이 존재해야 활성화가 된다고 하는데 해당 제품은 powder형태이며 이는 따로 용액이 필요합니다. 그렇다면 이 제품 안에 저 금속 이온이 함께 들어있는 건지, 아니면 Sigma 제품 중에 이 제품을 혼합할 수 있는 PBS가 따로 있는지(있다면 카탈로그 번호 알려주셨으면 합니다) 궁금합니다.

    1 answer

    1. Concanavalin A has 2 metal binding sites per monomer. Calcium and Manganese are required to bind these sites in order for sugar binding to occur. It is recommended to prepare stock solutions in PBS containing Ca2+ and Mn2+.

      See the link below to the product datasheet which includes preparation instructions:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/331/341/c0412dat.pdf

      Helpful?

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