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Merck

B3934

Biuret reagent

protein detection level 150-1,000 μg/mL

Synonym(s):

Micro Lowry, Onishi & Barr Modification

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
PubChem Substance ID:
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form

liquid

Quality Segment

protein detection level measuring range

150-1,000 μg/mL

storage temp.

2-8°C

General description

Biuret reagent is used for measuring total protein concentration. It comprises of copper II (cupric ion) alkaline solution, which interacts with the peptide bond to form a blue adduct which is measured at 540nm. Biuret method is less sensitive when compared to other protein estimation methods like Lowry and Folin-Ciocalteu techniques.

Application

Biuret reagent has been used for protein quantification of high-pressure thermal hydrolysis lysate(HPTH), oyster hemolymph and Clostridium cellulovorans (CBDclos) aggregates.


Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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