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ANTI-FLAG^® M2-Peroxidase (HRP) antibody, Mouse monoclonal

Name: ANTI-FLAG<SUP>®</SUP> M2-Peroxidase (HRP) antibody, Mouse monoclonal
Brand: SIGMA

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):

Monoclonal ANTI-FLAG^® M2, Anti-ddddk, Anti-dykddddk

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About This Item

NACRES:

NA.32

UNSPSC Code:

41106514

Conjugate:

peroxidase conjugate

Clone:

M2, monoclonal

Application:

ELISA (i)

Citations:

1904

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Properties

biological source

mouse

Quality Level

200

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

species reactivity

all

concentration

~1 mg/mL

technique(s)

indirect ELISA: 1:20,000

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... GPX1(2876)

Description

General description

The Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides.

Application

For simple, one-step detection by immunocytochemistry, ELISA, or Western blotting. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity.

Suggested dilution for immunocytochemistry and western blotting 1:100 to 1:1000
Suggested dilution for ELISA 1:20,000

Learn more product details in our FLAG^® application portal.

Physical form

Solution in phosphate buffered saline containing 50% glycerol plus preservative and stabilizer

Preparation Note

Dilute ANTI-FLAG M2-Peroxidase solution in Tris Buffered Saline (TBS): 0.05 M Tris, pH 7.4, with 0.15 M NaCl.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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This Item	A9469	F3040	SAB4301135
Sigma-Aldrich A8592 ANTI-FLAG^® M2-Peroxidase (HRP) antibody, Mouse monoclonal	Sigma-Aldrich A9469 Monoclonal ANTI-FLAG^® M2-Alkaline Phosphatase antibody produced in mouse	Sigma-Aldrich F3040 Monoclonal ANTI-FLAG^® M1 antibody	Sigma-Aldrich SAB4301135 Anti-FLAG Tag antibody produced in rabbit
species reactivity all	species reactivity all	species reactivity all	species reactivity all
clone M2, monoclonal	clone M2, monoclonal	clone M1, monoclonal	clone polyclonal
biological source mouse	biological source mouse	biological source (Bioreactor)	biological source rabbit
conjugate peroxidase conjugate	conjugate alkaline phosphatase conjugate	conjugate unconjugated	conjugate unconjugated
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody
technique(s) indirect ELISA: 1:20,000	technique(s) indirect ELISA: 1:20,000	technique(s) western blot: 10 μg/mL	technique(s) western blot: 1:1000-1:5000 (Cell Lysate)