A6063

Anti-Horse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): Rabbit Anti-Horse IgG (whole molecule)−AP
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162382
• Conjugate: alkaline phosphatase conjugate
• Clone: polyclonal
• Application: ELISA (d), WB
• Citations: 12

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• technique(s): direct ELISA: 1:30,000, western blot: 1:30,000
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Affinity isolated antibody is obtained from rabbit antiserum by immunospecific purification, which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to horse IgG. The horse IgG is present majorly in the serum, mucoasal surface, urinary tract, lungs and colostrum.

Horse IgGs have seven subclasses ranging from IgG1 to IgG7. Equine IgG antibodies mainly regulate mucosal and systemic immunological responses and thereby, provide protection against disease-causing pathogens such as Streptococcus equi., and the horse flu virus. Horse IgG may also function to control the advancement of EHV-1 infection . Anti-Horse IgG (whole molecule)-Alkaline Phosphatase antibody is specific for IgG in horses.

Immunogen

Horse IgG

Application

Anti-Horse IgG (whole molecule)-Alkaline Phosphatase antibody is suitable for use in direct ELISA (1:30,000) and western blot (1:30,000).

Anti-Horse IgG (whole molecule)-Alkaline Phosphatase antibody produced in rabbit has been used in indirect enzyme-linked immunosorbent assay (ELISA).

Binding of horse anti-diphtheria toxin IgG was analyzed by ELISA using alkaline phosphatase-conjugated rabbit anti-horse IgG.

Biochem/physiol Actions

The equine IgG subclasses elicit a strong respiratory burst by interacting with the interact with FcγR receptor peripheral blood leukocytes and with the Fc receptors on effector cells. It is useful as a monoclonal antibody in treating non-human primates (NHPs) infected with Ebola virus. It is used as a component in commercial equine IgG test called the SNAP Foal IgG test kit, for the diagnosis of failure of transfer of passive immunity (FTPI) in foals.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable