A5691
Anti-Actin, α-Smooth Muscle - Alkaline Phosphatase antibody, Mouse monoclonal
clone 1A4, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-Actin, α-Smooth Muscle, Monoclonal Anti-Actin, α-Smooth Muscle - Alkaline Phosphatase antibody produced in mouse, SMA
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All Photos(2)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
conjugate
alkaline phosphatase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
1A4, monoclonal
form
buffered aqueous glycerol solution
mol wt
antigen ~42 kDa
species reactivity
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil or appendix sections
western blot: 1:100 using chicken gizzard extract/ Mouse heart extract
isotype
IgG2a
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
mouse ... Acta2(11475)
rat ... Acta2(81633)
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General description
Actin is a highly conserved protein that is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle).
The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.
Immunogen
N-terminal synthetic decapeptide of α-smooth muscle actin.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
Immunocytochemistry was performed on kertocytes fixed in 1% PFA and incubated with mouse monoclonal anti-smooth muscle actin (14A) at a 1:100 dilution.
Immunocytochemistry was performed on smooth muscle cells from bovine aortas using the monoclonal anti-ACTA2 antibody. Cells were first grown on glass cover slips and fixed in 50% acetone/EtOH for 10 minutes at 4 degrees.
Mouse monoclonal anti-actin, α−smooth muscle-alkaline phosphatase antibody has been used for immunohistochemical application in mouse and human tissues.
Paraffin embedded sections of rat testis tissue grafts were immunohistochemically stained with mouse monoclonal anti-smooth muscle actin.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
Physical form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide as a preservative.
Other Notes
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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