This product is not charcoal treated. The datasheet is a general document for BSA products.
A4503
Bovine Serum Albumin
cold ethanol fraction, pH 5.2, ≥96%
Bovine Serum Albumin
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
CAS Number:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥96%
Form:
lyophilized powder
Technique(s):
ELISA: suitable, blood typing: suitable, microbiological culture: suitable
Impurities:
BT and VSV virus, tested
Pricing and availability is not currently available.
biological source
bovine
Quality Level
assay
≥96%
form
lyophilized powder
purified by
cold ethanol fractionation
origin
USA origin
technique(s)
ELISA: suitable, blood typing: suitable, microbiological culture: suitable
impurities
BT and VSV virus, tested
loss
≤5%
pH
5.2
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT Virus, none detected, VSV Virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
General description
Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.
Application
- Bovine Serum Albumin has been used in solution prepared for the isolation of heart mitochondria.
- It has been used in the cytotoxicity assay buffer.
- It has been used for the induction of protein overload nephropathy in rats.
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Preparation Note
Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
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This Item | |||
|---|---|---|---|
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| technique(s) ELISA: suitable, microbiological culture: suitable, blood typing: suitable | technique(s) immunocytochemistry: suitable | technique(s) blood typing: suitable | technique(s) cell culture | mammalian: suitable |
| assay ≥96% | assay ≥98% | assay ≥96% | assay ≥98% |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Sensitization of tumor cells to NK cell-mediated killing by proteasome inhibition.
Hallett WH et al.
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Cell reports, 24(10), 2614-2628 (2018-09-06)
Multiple pathways regulate the repair of double-strand breaks (DSBs) to suppress potentially dangerous ectopic recombination. Both sequence and chromatin context are thought to influence pathway choice between non-homologous end-joining (NHEJ) and homology-driven recombination. To test the effect of repetitive sequences
Rapamycin worsens renal function and intratubular cast formation in protein overload nephropathy.
Coombes JD et al.
Kidney International, 68, 2599-2599 (2005)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A4503-50G | 04061835553402 |
| A4503-5KG | 04061833458358 |
| A4503-1KG | 04061835553389 |
| A4503-100G | 04061835517619 |
| A4503-500G | 04061835553396 |
| A4503-10KG | 04061833693216 |
| A4503-10G | 04061835504480 |
| A4503-5G | 04061833693223 |
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The data sheet lists several methods for further purification of BSA after ethanol precipitation but does not say which of these were used for A4503. Is A4503 charcoal treated? The data sheet is the same for other BSA powders (e.g., A2153).
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Is this product biotin-free?
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This product is not tested for the presence of biotin. However, Albumin products prepared by cold ethanol fractionation are expected to be biotin-free.
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Bonjour y a t-il un risque l'altération du produit si la bovin sérum albumine a été conservée pendant 15 heures en dehors du frigo dans une pièce a température ambiante ?
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Product A4503 (Bovine Serum Albumin) will not spoil following storage for 15 hours at ambient room temperature.
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can A4503 product be used for primary neuron preparation?
1 answer-
This product has not been evaluated for mammalian cell culture. Please see the datasheet for the recommended applications for this product:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/384/276/a4503dat.pdfHelpful?
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Is Product No. A4503, Albumin from bovine serum, tested and found to be endotoxin-free?
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No. If you need such a grade, we would recommend Product No. A8806.
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Do you offer a kit for conjugating ligands to bovine serum albumin (Product A4503)?
1 answer-
Yes. It is our Product No. MBK1.
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Is Product No. A4053, Albumin from bovine serum, sterile? If not, how would recommend sterilizing it?
1 answer-
This product is not necessarily sterile. Solutions of albumin cannot be autoclaved, so in order to sterilize it we would recommend that it be dissolved in the appropriate medium (water, saline or buffered saline), followed by sterile filtration through a low protein-binding membrane.
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Is Product No. A4503, Albumin from bovine serum, tested and found to be protease-free?
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No. If you need such a grade, we would recommend Product No. A3059.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product No. A4503, Albumin from bovine serum, of US origin?
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It is typically from animals born and raised in the US. You may confirm this information by downloading the Certificate of Origin (CofO) for any lot of A4503 from our website. From the product detail page, simply type in the lot number in the box below the words Certificate of Origin, and click GO.
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