Undiluted aliquots of this product can be stored at -80º or -20º C for long-term storage. Under aseptic conditions, this product remains stable at 4°C until the CoA-listed retest date.
A1595
Bovine Serum Albumin solution
10% in DPBS, low endotoxin, fatty acid free, suitable for cell culture, sterile-filtered
Synonym(s):
Albumin solution from bovine serum, BSA solution
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About This Item
Biological source:
bovine
Assay:
90-120 mg/mL protein basis (biuret)
Form:
solution
Technique(s):
cell culture | mammalian: suitable
Impurities:
≤0.05% fatty acid, ≤200 EU/mL endotoxin
Concentration:
10% in DPBS
Pricing and availability is not currently available.
biological source
bovine
Quality Segment
sterility
sterile-filtered
product line
BioReagent
assay
90-120 mg/mL protein basis (biuret)
form
solution
purified by
heat shock fractionation
contains
does not contain stabilizer or preservative
origin
USA origin
concentration
10% in DPBS
technique(s)
cell culture | mammalian: suitable
impurities
≤0.05% fatty acid, ≤200 EU/mL endotoxin
pH
7.1-7.7
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
Application
Recommended for use as a base for the preparation of defined, fatty acid supplements. Can be loaded with up to 4 moles of fatty acids per mole of albumin.
BSA is a known fatty acid (FA) carrier. Thus in FA-related applications like cell culture, the specific BSA can be important, as related to control over specific fatty acids used to culture cell lines, because different cell lines can be variably sensitive to particular FA;s. Fatty acid-free BSA therefore is useful in cell culture work where the specific fatty acid content must be carefully controlled, so that researchers can control the specific FA′s needed for their particular cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific fatty acids that researchers wish to use in their particular cell culture experiments. Use of fatty-acid-free BSA also addresses concerns about endogenous FA′s potentially in non-fatty acid-free BSA.
BSA is a known fatty acid (FA) carrier. Thus in FA-related applications like cell culture, the specific BSA can be important, as related to control over specific fatty acids used to culture cell lines, because different cell lines can be variably sensitive to particular FA;s. Fatty acid-free BSA therefore is useful in cell culture work where the specific fatty acid content must be carefully controlled, so that researchers can control the specific FA′s needed for their particular cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific fatty acids that researchers wish to use in their particular cell culture experiments. Use of fatty-acid-free BSA also addresses concerns about endogenous FA′s potentially in non-fatty acid-free BSA.
Features and Benefits
- Fatty acid free / tested to be low in fatty acid content
- Low endotoxin
- Sterile-filtered
Preparation Note
Prepared with Heat Shock Fractionated fatty acid-free albumin in DPBS D5652.
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This Item | |||
|---|---|---|---|
| description 10% in DPBS, low endotoxin, fatty acid free, suitable for cell culture, sterile-filtered | description 5% in 0.85% sodium chloride, aseptically filled | description 30% in saline, high avidity, contains azide and caprylate, aseptically filled | description 30% in saline, protease free, aseptically filled |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) blocking: suitable | technique(s) ELISA: suitable, electrophoresis: suitable, western blot: suitable |
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| assay 90-120 mg/mL protein basis (biuret) | assay 4.8-5.5% protein basis (biuret) | assay 28-32% (biuret) | assay 28-32% protein basis (biuret) |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| form solution | form solution | form solution | form solution |
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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Can I store undiluted aliquots of this solution at -80º or -20º C for longterm storage? If not, how long does this solution last at 2-8ºC?
1 answer-
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How should we store this reagent?
1 answer-
As stated in the properties section of the product page, the storage temperature is 2-8°C
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