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5-Carboxyfluorescein

Name: 5-Carboxyfluorescein
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥95% (HPLC)

Synonym(s):

5-FAM

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About This Item

Empirical Formula (Hill Notation):

C₂₁H₁₂O₇

CAS Number:

76823-03-5

Molecular Weight:

376.32

UNSPSC Code:

12171500

NACRES:

NA.32

PubChem Substance ID:

57653195

MDL number:

MFCD00036874

Beilstein/REAXYS Number:

57037

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Properties

product line

BioReagent

Quality Segment

100

assay

≥95% (HPLC)

form

powder

solubility

DMF: soluble, DMSO: soluble, H₂O: soluble

fluorescence

λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35

InChI

1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

NJYVEMPWNAYQQN-UHFFFAOYSA-N

Description

General description

5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Application

5-Carboxyfluorescein has been used as a payload to study leakage phenomena on sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers

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This Item	21877	21879	38132
Sigma-Aldrich 86826 5-Carboxyfluorescein	Sigma-Aldrich 21877 5(6)-Carboxyfluorescein	Sigma-Aldrich 21879 5(6)-Carboxyfluorescein diacetate	Sigma-Aldrich 38132 N-(5-Fluoresceinyl)maleimide
description BioReagent, suitable for fluorescence, ≥95% (HPLC)	description BioReagent, suitable for fluorescence, ≥95% (HPLC)	description BioReagent, suitable for fluorescence, ≥90.0% (HPLC)	description suitable for fluorescence, ≥90% (HPLC), BioReagent
suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence, corresponds for coupling to thiols
fluorescence λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0	fluorescence λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0	fluorescence λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0 (esterase)	fluorescence λ_ex 490 nm; λ_em 518 nm in 0.1 M Tris pH 8.0
solubility DMF: soluble, DMSO: soluble, H₂O: soluble	solubility -	solubility DMSO: soluble	solubility -
form powder	form powder	form powder	form -
assay ≥95% (HPLC)	assay ≥95% (HPLC)	assay ≥90.0% (HPLC)	assay ≥90% (HPLC)
Quality Level 100	Quality Level 200	Quality Level 100	Quality Level 100

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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