52583

Lipase B Candida antarctica immobilized on Immobead 150, recombinant from yeast

≥2000 U/g

• Synonym(s): Candida antarctica Lipase
• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ≥2000 U/g
• Recombinant: expressed in yeast

Properties

• recombinant: expressed in yeast
• Quality Level: 100
• form: beads
• specific activity: ≥2000 U/g
• mol wt: 33 kDa
• storage temp.: 2-8°C

Description

General description

Lipase B or triacylglycerol ester hydrolases belongs to the class of hydrolases. Lipase B from Candida antarctica (CALB) possesses 317 amino acids and a molecular weight of 33 kDa. CALB has a catalytic triad and an open solvent accessible active site. The catalytic triad is usually Ser-His-Asp/Glu which is found in the carboxy terminal of parallel β sheet.

Application

Lipase B Candida antarctica immobilized on Immobead 150, recombinant from yeast has been used:

• in esterification reaction of lauric acid with n-butanol in a biphasic solvent system
• in hydrolysis of fish oil triglycerides
• in screening of enzymes for Morita–Baylis–Hillman (MBH) reaction

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Lipase B from Candida antarctica has been shown to be an effective catalyst for the synthesis of esters of ethyl D-glucopyranoside from fatty acids larger than octanoic acid. It has also been found to catalyze a wide variety of organic reactions including many different regio- and enantio-selective syntheses.

Lipases B from Candida antarctica (CALB) is a versatile catalyst for biotransformation reactions. In aqueous media, CALB behaves like an esterase rather than a lipase.

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Other Notes

1 U corresponds to the amount of enzyme which liberates 1 μmol butyric acid per minute at pH 7.5 and 40°C (tributyrin, Cat. No. 91010, as substrate)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)