356M-1

Cytokeratin 5 & 6 (D5 & 16B4) Mouse Monoclonal Antibody

• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: D5 & 16B4, monoclonal
• Application: IHC (p)
• Citations: 10

Properties

• biological source: mouse
• Quality Segment: 100, 500
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: D5 & 16B4, monoclonal
• description: For In Vitro Diagnostic Use in Select Regions (See Chart)
• form: buffered aqueous solution
• species reactivity: human
• packaging: vial of 0.1 mL concentrate (356M-14), vial of 0.5 mL concentrate (356M-15), bottle of 1.0 mL predilute (356M-17), vial of 1.0 mL concentrate (356M-16), bottle of 7.0 mL predilute (356M-18)
• manufacturer/tradename: Cell Marque^®
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:200
• isotype: IgG1
• control: mesothelioma
• shipped in: wet ice
• storage temp.: 2-8°C
• visualization: cytoplasmic
• Gene Information: human ... KRT5(3852)

Description

General description

Anti-CK 5 & 6 positivity is seen in 65% to 100% of epithelioid mesotheliomas, in 0% to 19% of lung adenocarcinomas, and in 22% to 80% of serous carcinoma of the ovary and peritoneum. Keratin 5 & 6 expression in lung adenocarcinoma is not common, and when it occurs, the reaction is usually confined to small focal areas or scattered cells. The most probable cause of this finding is the presence of squamous differentiation of lung adenocarcinoma. Anti-CK 5 & 6 positivity has been useful in recognizing squamous cell carcinoma of the skin. Less than 10% of carcinomas of the breast, colon, and prostate stain positively for this marker. Anti-CK 5 & 6 has also been used successfully as a myoepithelial cell marker in the prostate and breast to determine malignancy. Anti-CK 5 & 6 is a useful marker to distinguish lung squamous cell carcinoma from lung adenocarcinoma and large cell carcinoma within a panel including antibodies against TTF-1, Napsin A, p63, SOX-2, desmocollin3, and desmoglein3.

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Analysis Note

IVD

IVD

IVD

RUO

Other Notes

Cytokeratin 5 & 6 Positive Control Slides, Product No. 356S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

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Legal Information

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany