The molecular weight of this product is not determined. This material is produced by mechanical disruption, no chemical or enzymatic treatment has been applied. The estimated molecular weight range of this DNA is 50,000 – 100,000 Da and is primarily double stranded. Note that whole DNA from salmon sperm has a molecular weight of 1.98x10e11 Da. While the number of base pairs(bp) is not determined on a lot-to-lot basis, based on a approximate molecular weight of 650 Da per bp, the estimated chain length range is 77 – 154 bp. However, historical records indicate chain lengths up to 300 bp have been detected.
31149
Deoxyribonucleic acid, low molecular weight from salmon sperm
Synonym(s):
Salmon sperm dsDNA, low molecular weight DNA, DNA
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About This Item
CAS Number:
UNSPSC Code:
41106310
eCl@ss:
32160414
EC Number:
309-566-6
NACRES:
NA.52
MDL number:
Biological source:
fish
Form:
lyophilized powder
Solubility:
50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow
Storage temp.:
2-8°C
Pricing and availability is not currently available.
biological source
fish
grade
Molecular Biology, puriss. p.a.
form
lyophilized powder
impurities
≤5% protein
solubility
50 mM Tris pH 8.0: 10 mg/mL, clear, faintly brown-yellow
absorbance ratio
A260/280 nm ~1.4
storage temp.
2-8°C
Application
Various concentrations of Deoxyribonucleic acid, low molecular weight, from salmon sperm has been used:
- to study DNA binding to silicon-substituted hydroxyapatite nanocrystals.
- in simple electrochemical DNA biosensors to detect and evaluate DNA damage caused by UV-C radiation and reactive oxygen species.
- as a precursor in the synthesis of the bio-dots.
Biochem/physiol Actions
Salmon sperm DNA is often used as a blocking agent invarious research applications. It isknown to improve the specificity and sensitivity of the multi-AP assay byreducing non-specific binding and background signal.
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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What is base pair size of 31149-10G-F?
1 answer-
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What is the approximate estimate of guanine bases in % like 50% or more?
1 answer-
Produced by mechanical shearing of native salmon sperm DNA, this product has not undergone empirical determination of its exact guanine content. However, published literature indicates a typical salmon sperm DNA guanine base content of ~20.8 to 22%.
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Is this product double stranded and is it suitable as a substrate for nuclease activity assays?
1 answer-
This product is a double-stranded DNA. However, the DNA product D1501 has been successfully utilized as a substrate in the Enzymatic Assay of Deoxyribonuclease I and may be better suited for this application. For more information, refer to: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/291/147/d4527enz.pdf and https://www.sigmaaldrich.com/en/product/sigma/d1501.
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For the product 31149-50G-F (31149-50G-F), what is the molecular weight, or, the amount of base pairs? Thank you!
1 answer-
The molecular weight of this product is not determined. This material is produced by mechanical disruption, no chemical or enzymatic treatment has been applied. The estimated molecular weight range of this DNA is 50,000 – 100,000 Da and is primarily double stranded. Note that whole DNA from salmon sperm has a molecular weight of 1.98x10e11 Da. While the number of base pairs(bp) has not been investigated, based on a approximate molecular weight of 650 Da per bp, the estimated chain length range is 77 – 154 bp.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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