XTGHP-RO
Roche
X-tremeGENE™ HP DNA Transfection Reagent
High-performance polymer reagent for transfecting many cell lines
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About This Item
UNSPSC Code:
41106502
NACRES:
NA.55
grade
for molecular biology
Quality Level
form
liquid (aqueous solution)
usage
mL (suitable for 165 transfections)
packaging
pkg of 0.4 mL (06366244001)
pkg of 1.0 mL (06366236001)
pkg of 5 × 1 mL (06366546001)
manufacturer/tradename
Roche
technique(s)
transfection: suitable
storage temp.
−20°C
General description
X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.
Features and Benefits
X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116).
- Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 μm pore size membrane, and active in serum-containing medium.
- Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents.
- Generate physiologically relevant data using a reagent with low cytotoxic effects.
- Increase experimental throughput and enable target evaluation using a simple and consistent protocol.
Quality
Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.
Physical form
Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
X-tremeGENE is a trademark of Roche
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
334.4 °F
Flash Point(C)
168 °C
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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